What is the purpose of the Cytofix Cytoperm?

Description. This kit enables the fixation and permeabilization of cells which is necessary for staining intracellular cytokines with fluorochrome-conjugated anti-cytokine antibodies. The kit provides two reagents, fixation/permeabilization solution and BD Perm/Wash™ Buffer.

What is Cytofix Cytoperm?

Description. BD Cytofix/Cytoperm™ solution can be used for the simultaneous fixation and permeabilization of cells prior to intracellular cytokine staining. Preparation and Storage. Store undiluted at 4° C. Cytofix/Cytoperm solution is supplied as a 1X solution.

What is permeabilization buffer?

Product Description. Permeabilization Buffer is a ready to use permeabilization buffer for intracellular immunofluorescence staining for microscopy or flow cytometry. Ready-to-use buffered detergent solution. Permeabilize fixed cells for microscopy and flow cytometry applications.

What does it mean to permeabilize a cell?

Permeabilization. The permeabilization step removes more cellular membrane lipids to allow large molecules like antibodies to get inside the cell.

How does BD cytofix / cytoperm solution kit work?

BD Cytofix/Cytoperm™ Fixation/Permeabilization Solution Kit Enables the fixation and permeabilization of cells which is necessary for staining intracellular cytokines with fluorochrome-conjugated anti-cytokine antibodies

What is the purpose of BD cytofix fixation buffer?

Intended to preserve human and rodent lymphoid cells for subsequent immunofluorescent staining of intracellular cytokines Used to preserve light-scattering characteristics and fluorescence intensities of human and rodent hematopoietic cells that have been stained by immunofluorescence for subsequent flow cytometric analysis

How to stain for intracellular cytokines in bd-554722?

Wash cells two times in a buffer that contains a cell permeabilizing agent such as saponin (BD Perm/Wash™ buffer, Cat. 554723, which can be used as the wash buffer and as the antibody diluent). 2. Stain for Intracellular Cytokines a.

How to stain fixed cells for intracellular cytokines?

2. Stain for Intracellular Cytokines a. Thoroughly resuspend fixed/permeabilized cells in 50 µl of a saponin-containing buffer (e.g., BD Perm/Wash buffer) containing a pre- determined optimal concentration of a fluorochrome-conjugated anti-cytokine antibody or appropriate negative control. Incubate at 4°C for 30 minutes in the dark.