How do you dilute IDT primers?

To obtain a 100 µM solution, multiply # nmol x 10. That will equal the # µL to use for resuspension. For example: 20 nmol X 10 = 200 µL.

How do you reconstitute IDT oligos?

Tips for resuspending and diluting your oligonucleotides

1. During the dry-down process, oligos form a white flakey pellet at the bottom of the tube.
2. If resuspension is difficult, try heating the oligo at 55°C for 1–5 minutes, then vortex thoroughly.
3. IDT oligonucleotides (both DNA and RNA) are typically shipped dry.

How do you dilute nucleic acids?

If the purified genomic or plasmid DNA is too dilute for the selected downstream application, DNA may be concentrated by isopropanol precipitation.

1. Add 0.7 volumes of room temperature isopropanol to the purified sample.
2. Vortex, then spin for 15 min at 5,000 × g at 4 °C.

How to calculate the dilution of a drug?

The Tocris dilution calculator is based on the following equation: Concentration (start) x Volume (start) = Concentration (final) x Volume (final) This equation is commonly abbreviated as: C 1V 1 = C 2V 2.

What is the equation for the Tocris dilution calculator?

The Tocris dilution calculator is based on the following equation: This equation is commonly abbreviated as: C1V1 = C2V2 What volume of a given 10 mM stock solution is required to make 20ml of a 50 μ M solution? Using the equation C 1 V 1 = C 2 V 2, where C 1 =10 mM, C 2 =50 μ M, V 2 =20 ml and V 1 is the unknown:

What do you need to know about IDT reagents?

Webinar summary: Learn about IDT’s high-quality line of genomic reagents that can be used to facilitate your research of COVID-19, caused by the novel coronavirus, 2019-nCoV (officially named SARS-CoV-2). Get up to date. Stay up to date.

Which is an example of a dilution equation?

Concentration (start) x Volume (start) = Concentration (final) x Volume (final) This equation is commonly abbreviated as: C 1 V 1 = C 2 V 2. An example of a dilution calculation using the Tocris dilution calculator. What volume of a given 10 mM stock solution is required to make 20ml of a 50 μ M solution?

https://www.youtube.com/watch?v=hB8YSgE97H0