Other

What is sephacryl column?

What is sephacryl column?

HiPrep Sephacryl S-200 HR (high-resolution) size exclusion chromatography columns are designed for routine protein purification at laboratory scale. Convenient: Pre-packed Sephacryl columns are easily connected to ÄKTA systems and other chromatography systems for automated chromatography at low to medium pressure.

How do I choose a gel filtration column?

Choosing a Column for your Gel Filtration Experiment

  1. Size of the Analyte. First, keep its size in mind when choosing a column for purifying your analyte.
  2. Sample Purity. Okay, so you chose your column based on the size of your analyte.
  3. Sample Volume.
  4. Bead Size.
  5. Experimental Objectives.
  6. Column Efficiency.
  7. Further Reading.

How do you choose a SEC column?

How to Choose the Right SEC Column for Your Analysis

  1. Pore size and column dimension is based on the complexity of sample to be separated.
  2. LC System dispersion used for the analysis, as well as sample throughput.
  3. Based on these criteria, the most appropriate UPLC, UHPLC, or HPLC offering can be selected.

How does Column length affect gel filtration chromatography?

Increase in column length increases the resolution and increase in column diameter results in high bed volume and hence higher column capacity. The fractionation range and the exclusion limit can be controlled by varying pore size. The smaller the particle size of the gel, the higher the resolution achieved.

What can Sephacryl S-200 HR be used for?

Sephacryl S-200 High Resolution resin is designed for purifying antibodies, serum proteins and mid-size proteins (fractionation range globular proteins 5 × 10³ – 2.5 × 10⁵). BioProcess resin supported for industrial applications and well-established in approved processes.

What can Sephacryl gel filtration resin be used for?

Sephacryl High Resolution size exclusion chromatography resins allow fast and reproducible purification of proteins, polysaccharides, and other macromolecules by size exclusion chromatography at laboratory and industrial scale. Cost-efficient gel filtration resins providing high resolution with fast and easy operation.

What makes Sephacryl a high resolution copolymer?

The matrix of Sephacryl High Resolution resins is a cross-linked copolymer of allyl dextran and N,N’-methylene bisacrylamide. This crosslinking gives good rigidity and chemical stability. The narrow particle size distribution, together with steep selectivity curves, results in good preparative characteristics with maintained high resolution.

Which is the best resin for gel filtration?

BioProcess resin supported for industrial applications and well-established in approved processes. Sephacryl High Resolution chromatography resins is a highly versatile gel filtration resins that offers a wide range of fractionation capabilities.