How many kDa is a FLAG-tag?

1 kDa
FLAG is a short, 8 amino acid sequence DYKDDDDK about 1 kDa in size (D = aspartic acid, Y = tyrosine, K = lysine). Due to its small size, some researchers may choose to leave the tag intact, but cleavage can be performed with enterokinase.

What is a 3X FLAG-tag?

General description. The 3X FLAG Peptide is a synthetic peptide of 23 amino acid residue. The Asp-Tyr-Lys-Xaa-Xaa-Asp motif is repeated three times in the peptide. Eight amino acids at the C-terminus make up the classic FLAG sequence (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys).

What is FLAG-tag sequence?

The Flag® tag, also known as the DYKDDDDK-tag, is a popular protein tag that is commonly used in affinity chromatography and protein research for over 20 years now (6,7,8,9,10,11). As its second name suggests the tag consists of an amino acid sequence DYKDDDDK. (D=Aspartic acid; K=Lysine; Y=Tyrosine).

What is Flag Gene?

FLAG-tag, or FLAG octapeptide, or FLAG epitope, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology, having the sequence motif DYKDDDDK (where D=aspartic acid, Y=tyrosine, and K=lysine).

What does a flag tag do?

FLAG Tag Definition The FLAG tag allows highly specific pull-downs that contain low nonspecific background. A FLAG-tag can be used in many different assays that require recognition by an antibody.

How big is his tag?

His-tags. Molecular Weight: 0.2–1.6 kDa. 6x-His tag is 0.8 kDa.

What is the Pinnie rule?

Quick Rules Each player is given a pinnie which they must wear as a “tail” on the side of their body (with at least 75% of the pinnie being visible). Players tag other players by removing their “tail”. If a player successfully removes the “tail” of another player, they give the pinnie back to that player.

What is Flag tag used for?

The FLAG tag (peptide sequence DYKDDDDK) is a short, hydrophilic protein tag commonly used in conjunction with antibodies in protein pull-downs to study protein–protein interactions.

What is Flag tag antibody?

The FLAG tag (peptide sequence DYKDDDDK) is a short, hydrophilic protein tag commonly used in conjunction with antibodies in protein pull-downs to study protein–protein interactions. We offer quality antibodies specific to FLAG tag that can be used in a variety of research needs.

How do you purify the flag tagged protein?

How to Purify FLAG-tagged Proteins? FLAG-tagged recombinant protein can be affinity purified directly from a cell culture lysate or supernatant. The FLAG-tagged protein binds to the FLAG-tag specific monoclonal antibody conjugated on an agarose gel.

How many his in His tag?

A polyhistidine-tag is an amino acid motif in proteins that typically consists of at least six histidine (His) residues, often at the N- or C-terminus of the protein.

Why use a His tag?

One of the most commonly used tags is the polyhistidine tag, also known as His-Tag, which is a string of usually between six and nine histidine residues (see Figure 1 below). This method of tagging is especially useful as it allows for easy purification and detection of the recombinant protein.

How big is the size of a FLAG tag?

That brings the total size of the tag to 1012.9 dalton or roughly 1 kDa (1). The Flag-tag can be added either to the N-terminus or the C-terminus of a protein (1), respectively. Additionally it can be implemented in a tandem formation, meaning multiple Flag-tags can be used right next to each other (-DYKDDDDK-DYKDDDDK-DYKDDDDK).

What is the yield of a FLAG tag?

The typical protein yield per ml Flag® tag affinity resin is usually between 0.6-1 mg/ml, which is low, comparatively to the GST or the Rho1D4 tag. Typically the Flag® tag is used for protein purification from mammalian expression systems or general immunostaining and immunoprecipitation assays.

How is a FLAG tag added to a protein?

FLAG-tag. FLAG-tag, or FLAG octapeptide, or FLAG epitope, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology, having the sequence motif DYKDDDDK (where D= aspartic acid, Y= tyrosine, and K= lysine ). It is one of the most specific tags and it is an artificial antigen to which specific,

What are the alternatives to the FLAG tag?

Alternatives to the Flag®-tag The Flag® tag, also known as the DYKDDDDK-tag, is a popular protein tag that is commonly used in affinity chromatography and protein research for over 20 years now (6,7,8,9,10,11). As its second name suggests the tag consists of an amino acid sequence DYKDDDDK.