When to use Invitrogen TRIzol as a reagent?

Invitrogen™TRIzol Reagent is a ready-to-use reagent, designed to isolate high quality total RNA (as well as DNA and proteins) from cell and tissue samples of human, animal, plant, yeast, or bacterial origin, within one hour.

How is TRIzol used for RNA isolation in PCR?

The Invitrogen Life Technologies TRIzol Reagent (Total RNA Isolation Reagent) is a ready-to-use reagent for the isolation of total RNA from cells and tissues for use in PCR analysis. TRIzol reagent is a mono-phasic solution of phenol and guanidine isothiocyanate. During tissue homogenization or lysis, the TRIzol

How big of a sample does TRIzol reagent take?

TRIzol® Reagent performs well with small quantities of tissue (50–100 mg) and cells (5 × 10 6 ), as well as with large quantities of tissue (≥1 g) and cells (>10 7 ), and comes with prototcols for purification from samples of human, animal, plant, or bacterial origin.

Which is the Invitrogen total RNA isolation reagent?

I. PRINCIPLE The Invitrogen Life Technologies TRIzol Reagent (Total RNA Isolation Reagent) is a ready-to-use reagent for the isolation of total RNA from cells and tissues for use in PCR analysis. TRIzol reagent is a mono-phasic solution of phenol and guanidine isothiocyanate.

How is RNA homogenized in TRIzol reagent?

After homogenizing the sample with TRIzol® Reagent, chloroform is added, and the homogenate is allowed to separate into a clear upper aqueous layer (containing RNA), and interphase and red lower organic layers (containing the DNA and proteins). RNA is precipitated from the aqueous layer with isopropanol.

What is the melting temperature of a BGH primer?

Description It is a sequencing primer to sequence mammalian expression vectors having the BGH polyadenylation signal. Primer is supplied as 2 µg which equals 358 pMoles. The melting temperature is 54.8°C.