What does MACS stand for Miltenyi?

Magnetic-activated cell sorting
Magnetic-activated cell sorting (MACS) is a method for separation of various cell populations depending on their surface antigens (CD molecules) invented by Miltenyi Biotec.

What does miltenyi Biotec do?

Miltenyi Biotec is the world’s leading innovator in cellular research and its translation into clinical application. Our mission is to improve scientific understanding and medical progress by providing solutions that advance biomedical research and cellular therapy.

How are magnetic beads removed from cells?

There are three methods to remove the Dynabeads™ magnetic beads from the isolated cells: DETACHaBEAD™ Kits (positive isolation)—where the release agent is a polyclonal anti-Fab reagent outcompeting the binding of the bead-bound antibody on the cell, giving both antibody- and bead-free cells.

How does magnetic-activated cell sorting work?

How Magnetic-Activated Cell Sorting Works. MACS, also known as immunomagnetic cell separation, binds magnetic particles to cells through an antibody interaction with surface markers of the targeted cells. Then, those targeted cells are magnetically isolated from the rest of the biological sample.

What are the major differences between FACS and Macs?

The main benefit of MACS is its speed. Magnetic-activated cell sorting is 4-6 times faster than FACS. This allows researchers to run a higher quantity of samples in a shorter time. On the other hand, MACS has hidden costs associated with the storage and upkeep of necessary devices.

What is cell separation method?

Cell separation, also commonly referred to as cell isolation or cell sorting, is a process to isolate one or more specific cell populations from a heterogeneous mixture of cells. There are a number of cell separation methods available, each with its own pros and cons.

Who owns Miltenyi?

Miltenyi Biotec B.V. & CO. KG
Miltenyi Biotec/Parent organizations

Is Miltenyi Biotec a public company?

Miltenyi Biotec is a global biotechnology company headquartered near Cologne in Bergisch Gladbach, Germany….Miltenyi Biotec.

How does Immunomagnetic separation work?

Immunomagnetic separation (IMS) works using specific antigen-antibody interactions, similar to ELISA. However, rather than the confirmation of bacterial isolates or their toxins, IMS is frequently used to concentrate the target pathogen directly from seafood or seafood enrichments.

What is a Mac separator?

MACS Separators are strong permanent magnet that enable the isolation of any cell type. Together with MACS MicroBeads and MACS Columns, they deliver target cells with high purities and recoveries. Thereby, even cells that are only slightly labeled with MACS MicroBeads are retained in the column.

What is FACS technique?

Fluorescence-activated cell sorting (FACS) is a technique to purify specific cell populations based on phenotypes detected by flow cytometry. This method enables researchers to better understand the characteristics of a single cell population without the influence of other cells.

What is FACS buffer?

Flow Cytometry Staining Buffer (FACS Buffer) This basic FACS Buffer is a buffered saline solution that can be used for immunofluorescent. staining protocols, antibody and cell dilution steps, wash steps required for surface staining and flow cytometric analysis.

What are the features of Miltenyi Biotec cell separation?

Miltenyi Biotec offers i) a column-based technology that relies on nano-sized superparamagnetic MACS® MicroBeads and ii) a column-free technology using micro-sized superparamagnetic MACSxpress® Beads. Features of these cell separation techniques and the benefits over other technologies are explained in the following chapters.

How to contact Miltenyi Biotec for technical support?

For immediate technical support, use our live chat. As a global market leader with numerous subsidiaries and distributors, Miltenyi Biotec is committed to providing our customers around the world with the highest quality products.

How is magnetic field used in cell separators?

The cell separators contain powerful permanent magnets that induce a high-gradient magnetic field within MACS Columns – a field strong enough to retain large cell numbers labeled even minimally with MACS MicroBeads. Flexible and convenient options from small- to large-scale cell separations with up to eight separations in parallel.

How are microbeads used in magnetic cell separation?

Labeling of the REAlease Biotin Complex with Anti-Biotin MicroBeads allows for magnetic isolation of these cells. Following cell separation, both MicroBeads and REAlease Biotin Complex can be gently removed, leaving the cells bead- and label-free for further cell separation steps.