What is DTT chemical used for?

Thermo Scientific DTT (DL-Dithiothreitol; Clelands reagent) is used to stabilize enzymes and other proteins, which possess free sulfhydryl groups. It has been shown to restore activity lost by oxidation of these groups in vitro.

What is DTT in DNA extraction?

DTT is used as a reducing or “deprotecting” agent for thiolated DNA. The terminal sulfur atoms of thiolated DNA have a tendency to form dimers in solution, especially in the presence of oxygen. Dimerization greatly lowers the efficiency of subsequent coupling reactions such as DNA immobilization on gold in biosensors.

What is the role of DTT in protein extraction?

DTT is frequently used to reduce the disulfide bonds of proteins and peptides. It prevents intramolecular and intermolecular disulfide bonds from forming between cysteine residues of proteins.

What is the molecular weight of DTT?

154.253 g/mol
Dithiothreitol/Molar mass

How is DTT used in Thermo Scientific Research?

Learn more Thermo Scientific DTT (DL-Dithiothreitol; Clelands reagent) is used to stabilize enzymes and other proteins, which possess free sulfhydryl groups. It has been shown to restore activity lost by oxidation of these groups in vitro. DTT quantitatively reduces disulfide bonds and maintains monothiols in a reduced state ( see Reference 1).

What is the chemical structure of dithiothreitol ( DTT )?

Dithiothreitol. DTT’s formula is C 4 H 10 O 2 S 2 and the chemical structure of one of its enantiomers in its reduced form is shown on the right; its oxidized form is a disulfide bonded 6-membered ring (shown below). The reagent is commonly used in its racemic form, as both enantiomers are reactive.

Can you use DTT powder in a solution?

• DTT is not stable in solution. Only freshly-made DTT solutions should be used. To prepare a 1 M DTT solution, dissolve 1.55 g of DTT powder in 10 mL of deionized water (e.g. Water, nuclease-free, #R0581).

What can DTT be used for in electrophoresis?

At a final 0.1 M concentration, DTT is also widely used for disruption of protein disulfide bonds in SDS-polyacrylamide gel electrophoresis. • Free of endo-, exodeoxyribonucleases, ribonucleases, and phosphatases. • DTT is not stable in solution.