Does Burkholderia cepacia grow on MacConkey Agar?

Burkholderia cepacia organisms grow well on standard solid media such as 5% sheep blood, chocolate, and MacConkey agars; in thioglycollate and brain-heart infusion broths; and in broth-based blood culture systems.

How do you test for Burkholderia cepacia?

To test for Burkholderia, ARL Bio Pharma prepares enrichment media (Tryptic Soy Broth), along with a specialized Burkholderia cepacia Complex Selective Agar. This special agar inhibits non-Burkholderia organisms, and changes color to indicate the likely presence of the target microorganisms.

What color is Burkholderia cepacia?

Typical colonies of Burkholderia cepacia are circular, and entire. Colour formation is based on natural pigment expression and colonies vary from grey to sage green, with the medium changing from orange to bright pink. Store the prepared plates of medium at 2-8°C. Good growth, grey colonies with bright pink medium.

What shape is Burkholderia cepacia?

Burkholderia cepacia are aerobic rod-shaped, free-living, motile Gram- negative bacteria ranging from 1.6- 3.2 μm. They have been found to possess multitrichous polar flagella as well as pili used for attachment.

What does E coli look like on a MacConkey agar plate?

For example, growth on a MacConkey agar plate shows that E. coli are not inhibited by bile salts and crystal violet. The pink color of the bacterial growth indicates E. coli can ferment lactose and tells you that it is a gram-negative bacterium.

What does Pseudomonas aeruginosa look like on MacConkey Agar?

In MacConkey agar, Pseudomonas aeruginosa forms flat and smooth colonies that are between 2 and 3mm in diameter. Generally, these colonies have regular margins and have an alligator skin-like appearance when viewed from above.

How long can you live with B. cepacia?

One-year survival was 67% for B. cepacia positive patients and 92% for B. cepacia negative patients. Recent modifications in antimicrobial and immunosuppressive therapy since 1995 have resulted in no deaths early post-transplant in the last five patients transplanted.

How do you get Burkholderia?

Research has shown that people with CF can get B. cepacia from others who are infected with these bacteria. The germs spread either by direct contact, such as kissing, or indirectly from touching objects with the germs, such as doorknobs.

Can B. cepacia be cured?

Burkholderia bacteria are often resistant to many antibiotics, which makes them difficult to treat once they infect the lungs. However, some species may be successfully treated with combinations of antibiotics. Basic infection prevention and control practices reduce the risk of getting or spreading B. cepacia.

Is there a cure for B. cepacia?

What are the symptoms of B. cepacia?

The symptoms of B. cepacia, if any, are the same as those for any lung infection and can include fever, cough, congestion, shortness of breath, and wheezing.

When to use Remel Burkholderia cepacia selective agar?

BURKHOLDERIA CEPACIA SELECTIVE AGAR (BCSA) INTENDED USE. Remel Burkholderia Cepacia Selective Agar (BCSA) is a solid medium recommended for use in qualitative procedures for the selective and differential isolation of Burkholderia cepacia complex from respiratory secretions of patients with cystic fibrosis.

What are the colors of Burkholderia cepacia colonies?

Re-incubate for a further 24 hours if necessary. Typical colonies of Burkholderia cepacia are circular, and entire. Colour formation is based on natural pigment expression and colonies vary from grey to sage green, with the medium changing from orange to bright pink.

How long do you incubate Burkholderia cepacia for?

Streak onto Burkholderia cepacia Medium and incubate at 37°C for 48 to 72 hours. Examine after 48 hours for sage green colonies and the medium turning from straw-green to bright pink. All colonies should be further identified and confirmed. Re-incubate for a further 24 hours if necessary.

What does b.cepacia look like on agar?

B. cepacia complex colonies will be yellow on this medium. Colonies that absorb the crystal violet dye will appear purple to purple-gray. Acid production from carbohydrate oxidation will create a yellow zone in the medium surrounding the agar while peptone utilization will result in a pink zone.