How does reticulin stain work?

Reticulin stains use silver and rely on the argyrophilic properties of the fibers. Argyrophilic cells can adsorb silver but cannot reduce it. Argyrophilic cells need a reducer/developer to convert the invisible silver salts to a visible metallic silver.

What stain is used for Reticulin?

Reticulin stain uses silver impregnation to detect reticulin fibers, which are made of type 3 collagen. The fibers appear black against a gray to light pink background.

Which method is used to demonstrate Reticulin Fibres?

The Gordon and Sweet’s silver staining method is used to demonstrate reticular (retic) fibers. This method relies on the impregnation of retic fibers with silver through oxidation and reduction. The tissue is first oxidized using potassium permanganate to enhance subsequent staining.

What are some pathologies that elastin stains can demonstrate?

Elastin, along with reticular fibers, another example of a connective tissue, are not easily distinguished with H&E and therefore require special stains. Pathologists may look at elastic fibers for diagnosis of Marfan Syndrome, emphysema, Giant Cell Arteritis, sun exposure, and more.

Is H&E a special stain?

H&E also serves as what is arguably the most popular background stain in immunohistochemistry (IHC). In most cases, special stains are used when the pathologist/researcher wants to differentiate and/or identify components observed in tissue sections previously stained with H&E.

How do you make reticulin stain?

Method

1. Deparaffinise sections with xylene then take through alcohols to water.
2. Oxidise in acidified potassium permanganate for 3 minutes.
3. Rinse in distilled water.
4. Decolourise with 2% oxalic acid for 1 min.
5. Rinse in distilled water.
6. Mordant in 4% iron alum for 10 minutes.
7. Rinse in distilled water.

What is reticulin made of?

Reticulin is a histological term used to describe a type of fiber in connective tissue composed of type 3 collagen in which these reticular fibers crosslink to form a fine meshwork.

What do reticular fibers look like?

Reticular tissue is a special type of connective tissue that predominates in various locations that have a high cellular content. It has a branched and mesh-like pattern, often called reticulum, due to the arrangement of reticular fibers (reticulin). These fibers are actually type III collagen fibrils.

What special care should be taken when using Van Gieson?

Wash with plenty of water. If skin irritation occurs: Get medical advice/attention. lungs. Get medical attention.

What stains pink in H&E?

eosin
What structures are stained pink (eosinophilic or acidophilic)? Most proteins in the cytoplasm are basic, and so eosin binds to these proteins and stains them pink. This includes cytoplasmic filaments in muscle cells, intracellular membranes, and extracellular fibres.

What staining is best for nucleus?

Staining the nucleus. The bulk of the content inside the nucleus is nucleic acid, so nucleic acid stains are the obvious choice for nuclear staining. There are two main types of nucleic acid stains: those that will pass through the cell membrane (i.e., cell permeant) and those that will not (i.e., cell impermeant).

How are reticulin stains used in connective tissue?

Reticulin stains use silver and rely on the argyrophillic properties of the fibers. Argyrophillic cells can adsorb silver but cannot reduce it. Adsorb = surface based process whereas absorb = permeation through the tissue. Argyrophillic cells need a reducer/developer to convert the invisible silver salts to a visible metallic silver. 1. 2. 3. 4.

How to make reticulin fiber stain with distilled water?

Procedure 1. Dewax and hydrate the preparation until it reaches the distilled water. 2. Place on the section 5 drops of Reagent A and 5 drops of Reagent B, leave to act 5 minutes. 3. Rinse with distilled water. 4. Place on the section 10 drops of Reagent C, leave to act for 3 minutes. 2 Version 1: JMBJUL17 CEIVD09EN 5. Wash with distilled water.

How is silver impregnated in a reticulin stain?

Reticulin fibers are agyrophilic, meaning that these tissue elements will stain black with a silver solution using the aid of a chemical reducer, which brings the silver into a visible form. This silver staining process is known as silver impregnation.

How to stain reticulin with Gordon and sweet?

1 Deparaffinise sections with xylene then take through alcohols to water. 3 Rinse in distilled water. 5 Rinse in distilled water. 7 Rinse in distilled water. 9 Rinse quickly in distilled water. 13 Rinse in distilled water.