Can you reuse Amicon Ultra centrifugal filters?
Can you reuse Amicon Ultra centrifugal filters?
As Ammara mentioned, yes they can be reused. We reuse the filters for the same protein(same sample if working with cell lysates).
How do you use Amicon Ultra 2 mL centrifugal filters?
To recover the concentrated solute, invert the Amicon® Ultra filter device and concentrate collection tube. Place in centrifuge and counterbalance with a similar device. Spin for 2 minutes at 1,000 × g to transfer the concentrated sample from the device to the tube.
How to use amicon ultra 15?
While most of the sample is filtered in the first 15 to 30 minutes of centrifugation, the lowest concentrate volume (150–300 μL) is reached after spinning for 15 to 60 minutes. Spin conditions: 4,000 × g, room temperature, 15 mL starting volume.
What is Amicon filter?
Amicon® ultrafiltration devices provide a fast, reliable method to concentrate proteins and other biomolecules, separate components in biological and environmental samples by molecular weight, and desalt and dialyze proteins and antibodies for sample preparation.
How are Amicon ultra 0.5 mL centrifugal filters used?
With their vertical membrane design, Amicon Ultra 0.5 mL filters deliver great performance and lower spin times for protein purification. Less Concentration of biological samples containing antigens, antibodies, enzymes, nucleic acids, or microorganisms
What is the MSD for Amicon Ultra 15?
Amicon® Ultra-15 Centrifugal Filter Unit MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents. Retrieving price…
Which is membrane are available in Amicon ultra units?
Select a Molecular Weight Cutoff that is 2x smaller that the Molecular Weight (MW) of the solute of interest. Which membranes are available in Amicon Ultra units? Amicon Ultra units are only available with Ultracel regenerated cellulose material. This high recovery/low binding membrane is offered in 3,000, 10,000, 30,000, 50,000 & 100,000 MWCO.
Can a Amicon ultra be used for PCR fragments?
Yes, Amicon Ultra units have been evaluated for use with Genomic and PCR fragments. The 30 kDa MWCO provides the best balance between recovery and spin time.