What is the restriction site for EcoRI?

Additional Information: The EcoRI is a restriction enzyme that creates four nucleotide sticky ends with the end of 5′. The enzyme cuts at the recognition site of G/AATTC which has a complementary sequence of CTTAA/G.

What are restriction sites in gel electrophoresis?

There exist an enzyme, called restriction enzyme, that can identify a particular nucleotide sequence, called restriction sites, and perform cleaving operation. This process separates genetic material into smaller fragments which may contain gene(s) of interest.

Is EcoRI methylation sensitive?

Although the EcoRI enzyme recognizing GAATTC is used as an enzyme that is not sensitive to methylation in MSAP, EcoRI did not digest GAATTmC sequences when cytosines at both strands are methylated [34,35].

How is Eco RI used in restriction modification?

Eco RI is a restriction enzyme that cleaves DNA double helices into fragments at specific sites. It is also a part of the restriction modification system . In molecular biology it is used as a restriction enzyme. Eco RI creates 4 nucleotide sticky ends with 5′ end overhangs of AATT.

What kind of restriction site does EcoRI use?

EcoRI cuts its restriction site with a preference for either smaller or larger size polynucleotide depending on the ionic strength of the medium.

What kind of sticky ends does Eco RI make?

The cut made by the Eco RI enzyme produces sticky ends on the vector mainly plasmids or viral DNA’s. ^ a bPingoud A, Jeltsch A (September 2001).

What are the reaction conditions for EcoRI restriction enzyme?

Thermo Scientific EcoRI restriction enzyme recognizes G^AATTC sites and cuts best at 37°C in its own unique buffer. See Reaction Conditions for Restriction Enzymes for a table of enzyme activity, conditions for double digestion, and heat inactivation for this and other restriction enzymes.