What is the meaning of NS1 reactive?

Interpretation of results A positive NS1 test result confirms dengue virus infection without providing serotype information. A negative NS1 test result does not rule out infection. People with negative NS1 results should be tested for the presence of dengue IgM antibodies to determine possible recent dengue exposure.

What does dengue NS1 antigen non reactive mean?

A negative or non-reactive result indicates absence of detectable dengue virus antibodies. However, a negative or non-reactive test result does not preclude the possibility of exposure to or infection with dengue virus.

What is difference between NS1 and IgM?

NS1 antigen is generally found during Day 1 and up to Day 9 after onset of fever. The detection of anti-NS1 is inhibited if anti-NS1 antibodies produced. Whereas IgM become detectable by Day 3 to Day 5 after onset of illness in primary dengue and by Day 1 to Day 2 after onset of illness in secondary infections.

What is NS1 in biology?

NS1 is a highly conserved non-structural protein among flaviviruses, which exist in diverse forms. The intracellular dimer form of NS1 plays role in genome replication, whereas, the secreted hexamer plays role in immune evasion.

How long is NS1 positive?

NS1 Testing Positive test for DENV may mean that NS1 antigen is typically detectable within 1 to 2 days following infection and up to 9 days following symptom onset. NS1 antigen may also be detectable during secondary dengue virus infection, but for a shorter duration of time (1-4 days following symptom onset).

What happens if dengue test is positive?

A positive result means you probably have been infected with the dengue virus. A negative result can mean you aren’t infected or you were tested too soon for the virus to show up in testing.

What happens if dengue IgM is positive?

Positive IgM and IgG tests for dengue antibodies detected in an initial blood sample mean that it is likely that the person became infected with dengue virus within recent weeks. IgM antibody tests can be positive if a person has been infected with a similar virus, such as chikungunya (called cross-reaction).

How long does dengue NS1 stay positive?

The presence of dengue NS1 antigen is consistent with acute-phase infection with dengue virus. Positive test for DENV may mean that NS1 antigen is typically detectable within 1 to 2 days following infection and up to 9 days following symptom onset.

How do you confirm dengue?

Doctors can diagnose dengue infection with a blood test to check for the virus or antibodies to it. If you become sick after traveling to a tropical area, let your doctor know. This will allow your doctor to evaluate the possibility that your symptoms were caused by a dengue infection.

What happens if IgM positive?

IgM is usually the first antibody produced by the immune system when a virus attacks. A positive IgM test indicates that you may have been infected or that you have recently been vaccinated and your immune system has started responding to the vaccination and that your immune system has started responding to the virus.

How soon can dengue be detected?

Results can take from four days to two weeks, according to the Centers for Disease Control and Prevention (CDC). Molecular tests of blood are not likely to detect the virus after 7 days of illness.

Can I get dengue without fever?

An overwhelming 75 – 90% of patients acquire dengue fever without any symptoms, not even fever.

How is the NS1 antigen test used in medicine?

Medical use. Dengue testing. The medical use of the NS1 antigen test can be defined to diagnose dengue infections and is effective to 1st day detection. Additionally, NS1 assay is useful for differential diagnostics in regards to flaviviruses.

When to test an antibody for cross reactivity?

This is often the case when two antigens have similar structural regions that the antibody recognizes. Cross-reactivity can invalidate the results of an experiment and thereby impact scientific reproducibility. Thus, testing an antibody for cross-reactivity with closely related proteins is a critical validation experiment.

Which is an example of immunologic cross reactivity?

Immunologic cross-reactivity occurs when adaptive immune response against one antigen also occurs to another antigen with amino acid structural similarity. Rather, we used biopsies from patients with intestinal spirochetosis to evaluate the potential of cross-reactivity of a commercially available polyclonal antibody directed against T pallidum.

What does DENV by NS1 antigen stand for?

DENV by NS1 antigen is laboratory confirmation of dengue in people also assessing clinical aspects (as well as, taking into account where the individual may have traveled recently).