Why is CMV a strong promoter?

Overall, our current study suggested that the CMV promoter, which provides strong and prolonged transcription from AAV vectors, elicits relatively low frequency of gene targeting when used in AAV-based targeting vectors to drive the NeoR gene.

What determines the strength of a promoter?

The specific sequence of the promoter determines the strength of the promoter (a strong promoter leads to a high rate of transcription initiation). The presence or absence of the protein will affect the strength of the promoter.

Is the ef1α promoter the same in humans?

Although, all are called the EF1α promoter, the functional activity from one commercially-obtained promoter was markedly lower in four different cell lines in vitro and in vivo in rat submandibular glands.

Is the EF1A core promoter as strong as CMV?

Not as strong as viral promoters like CMV. Tends not to get silenced (like CMV does). Sometimes the EF1a core promoter with CMV enhancer is used. Citing Invivogen “The EF-1 alpha gene encodes for elongation factor-1 alpha which is one of the most abundant proteins in eukaryotic cells and is expressed in almost all kinds of mammalian cells.

Which is a stronger promoter SV40 or EF1?

The promoter of this ’housekeeping’ gene exhibits a strong activity, higher than viral promoters such as SV40 and RSV promoters [1] and, on the contrary to the CMV promoter, yields persistent expression of the transgene in vivo [2]. InvivoGen has cloned the EF-1α promoter region of different species, among them the chimpanzee and rat.

Is the rtTA gene carried by the EF1A promoter?

In addition to the TRE lentivirus, we also transduced mammalian cells (or transfected Drosophila cells) with a vector carrying the rtTA gene driven by the EF1A promoter. This vector also carries neomycin resistance, which was used to select for transduced mammalian cells.