What is a peroxidase stain?

per·ox·i·dase stain a method for demonstrating peroxidase granules in some neutrophils and in eosinophils; the enzyme promotes the oxidation of benzidine by hydrogen peroxide; tissues treated with horseradish peroxidase can also have the enzyme detected in the electron microscope.

What is the peroxidase stain used for?

Use: The peroxidase reaction is one of the most important stains in hematology and is used for the recognition of myeloid differentiation in acute leukemia. The presence of peroxidase reactivity in >3% leukemic blasts is firm evidence of a myeloid origin.

What does MPO staining distinguish?

Myeloperoxidase (MPO) is present in granules of myeloid and monocytic cells, but absent from lymphocytes. Therefore MPO is an important marker for discriminating myeloid vs. lymphoid blasts. Staining is used to distinguish acute myeloid leukemia (AML) and other myeloid leukemias from lymphoid disorders.

What is cytochemical staining?

Cytochemical stains, which are enzymatic colorimetric reactions that occur in the cells of interest, were necessary to assign and confirm myeloid and lymphoid lineage. Nonetheless, cytochemical testing remains a useful adjunct assay for the proper classification of acute leukemia in a number of diagnostic settings.

What do you need to know about the peroxidase stain?

peroxidase stain. Also found in: Dictionary, Thesaurus, Encyclopedia. a method for demonstrating peroxidase granules in some neutrophils and in eosinophils; the enzyme promotes the oxidation of benzidine by hydrogen peroxide; tissues treated with horseradish peroxidase can also have the enzyme detected in the electron microscope.

How long does ABC peroxidase standard staining kit last?

ABC Peroxidase Standard Staining Kit is covered under our general 1-year warranty and is guaranteed to be fully functional for 12 months from the date of shipment, if stored as recommended (4 degrees C).

Which is more sensitive ABC peroxidase or regular senstivity?

The Ultra-Sensitive ABC Peroxidase Staining Kits are approximately five times more sensitive than the regular-senstivity kit, enabling more dilute primary antibodies to be used while producing equal staining intensity.

What kind of staining is used in Pierce ABC?

Pierce ABC Staining Kits are highly sensitive, produce very low background staining and have rapid avidin-biotin interactions. Standard Kits contain only the two essential reagents (avidin and biotinylated HRP) can be used with nearly any species of biotinylated secondary antibody.