Is folate involved in DNA synthesis?

Folic acid is an essential nutrient necessary for protein and nucleic acid synthesis (DNA and RNA).

How is folate used in DNA synthesis?

Folate, as 5,10 methylene THF, donates a methyl group to uracil converting it to thymine, which is used for DNA synthesis and repair.

How does folate affect DNA?

If folate is limited, imbalances in the DNA precursor pool occur, and uracil may be misincorporated into DNA. Subsequent misincorporation and repair may lead to double strand breaks, chromosomal damage and cancer. Moreover, folate affects gene expression by regulating cellular S-adenosylmethionine (SAM) levels.

Does folic acid make DNA?

Folic acid is the synthetic form of folate and it is found in supplements and in fortified food. It is important in periods of rapid cell growth and division such as pregnancy and infancy since it is essential for maintaining new cells and to make DNA and RNA.

Which is the second strand cDNA synthesis kit?

Invitrogen Second Strand cDNA Synthesis Kit is designed to produce double-stranded cDNA from the first-strand reaction without the need for intermediate organic extraction or ethanol precipitation steps. The convenient single-tube format speeds up the synthesis procedure and maximizes cDNA recovery.

Which is the primer for second strand synthesis?

Subsequent removal of the mRNA by alkaline hydrolysis leaves single-stranded cDNA molecules again with a small 3′ loop. This loop can be used by either reverse transcriptase or Klenow fragment of DNA polymerase I as a primer for second-strand synthesis.

Which is the classical scheme for cDNA synthesis?

The classical scheme for the synthesis of double-stranded cDNA as it was reported in 1976 is described. Reverse transcription of mRNA with oligo (dT) as the primer generates first strands with a small loop at the 3′ end of the cDNA (the end that corresponds to the 5′ end of the mRNA).

Do you need to purified TSO before 2 nd strand synthesis?

The TSO will function as a primer for 2 nd strand synthesis by primer extension. The RT reaction does not need to be purified prior to 2 nd strand cDNA synthesis. If a cDNA cleanup step is desired, we recommend AMPure XP or SPRIselect bead at a 1:2.5 sample to bead volume ratio.