What is chip based digital PCR?

Workflow of chip-based digital PCR (dPCR) is based on four stages: PCR reaction mix preparation, seal and load chips, PCR amplification and fluorescence detection. Data analysis with quality control was performed and aneuploidies status was determined.

Is digital PCR more sensitive than qPCR?

qPCR measures the accumulation of DNA during a PCR reaction. The increase in quantity of DNA at each cycle is measured by the change in intensity of a fluorescent signal. Digital PCR is a highly precise approach to sensitive nucleic acid detection and quantification.

What is qPCR and dPCR?

Quantitative PCR (qPCR) and digital PCR (dPCR) are both methods of determining the quantity of a particular nucleic acid target in a given reaction.

How is digital PCR used for nucleic acid detection?

Digital PCR for higher accuracy, sensitivity and absolute quantification. Digital PCR is a new approach to nucleic acid detection and quantification that offers an alternate method to conventional real-time quantitative PCR for absolute quantification and rare allele detection. Digital PCR works by partitioning a sample of DNA or cDNA…

What does Bio-Rad’s Droplet Digital PCR system do?

Bio-Rad’s Droplet Digital PCR System automates the ddPCR workflow of droplet generation, thermal cycling, droplet reading, and data analysis, making this technology accessible to the working research laboratory.

How does digital PCR improve TaqMan assays?

Digital PCR extends capabilities for advanced applications. Digital PCR instruments and reagents enhance the performance of existing TaqMan Assays, enabling applications that benefit from higher sensitivity, precision, and absolute quantification.

How is the quantification of dPCR carried out?

Quantification by dPCR hinges on the fact that the random distribution of molecules in many partitions follows a Poisson distribution. Each partition acts as an individual PCR microreactor and partitions containing amplified target sequences are detected by fluorescence.