What is anti V5 antibody?
What is anti V5 antibody?
V5 antibodies are immunological tools used to detect the V5 epitope tag. Derived from a small epitope found on the P and V proteins of the paramyxovirus of the simian virus 5 family, the V5 tag can vary in size from 9 to 14 amino acids.
How big is a V5 tag?
about 14 aa
The V5 tag is about 14 aa, which as far as I know should correspond roughly 1-1.5KDa difference in molecular weight.
What is a V5 tag?
The V5 tag is derived from a small epitope (Pk) found on the P and V proteins of the paramyxovirus of the simian virus 5 (SV5) family. V5 tag antibodies provide a dependable method for the detection and purification of tagged target proteins without a protein-specific antibody or probe.
What is an anti FLAG antibody?
General description. Anti Flag M2 antibody is used for the detection of Flag fusion proteins. This monoclonal antibody is produced in mouse and recognizes the FLAG sequence at the N-terminus, Met N-terminus, and C-terminus. The antibody is also able to recognize FLAG at an internal site.
What is the V5 tag?
How do you use epitope tags?
Epitope tagging is a method of expressing proteins whereby an epitope for a specific monoclonal antibody is fused to a target protein using recombinant DNA techniques. The fusion gene is cloned into an appropriate expression vector for the experimental cell type and host cells are transfected.
What is immunoprecipitation used for?
Immunoprecipitation is one of the most widely used methods for isolation of proteins and other biomolecules from cell or tissue lysates for the purpose of subsequent detection by western blotting and other assay techniques.
How big is a flag tag?
1012.0 Da
2. Alternatives to the Flag-tag
| Feature | Flag® Tag |
|---|---|
| Size of the tag in Da | 1012.0 Da |
| Affinity matrix | Anti Flag® antibody (approx. 150 kDa) |
| Elution conditions | Flag® peptide, low pH, or tag cleavage |
| Specificity of interaction (KD) | 100 nM (3) |
Why do we use epitope tagging?
Epitope tagging is a technique in which a known epitope is fused to a recombinant protein using genetic engineering. Epitope tags make it possible to detect proteins when no antibody is available. This technique can be used to characterize newly discovered proteins and low abundant proteins.
What are epitope tags for?
Epitope tagging of proteins. Epitope tags and their antibodies are used for detecting expression of novel, low abundance, or poorly immunogenic proteins for which no antibodies are available.
What is immunocytochemistry used for?
After the antibodies bind to the antigen in the cell sample, the enzyme or dye is activated, and the antigen can then be seen under a microscope. Immunocytochemistry is used to help diagnose diseases, such as cancer. It may also be used to help tell the difference between different types of cancer.
What is the difference between IP and co IP?
Difference between IP and co-IP is the focus of the experiment. IP is focused on the primary target, which binds the antibody. Whereas, Co-IP targets the secondary targets, which interacts with the primary proteins, instead of antibody.
Is there an antibody against the V5 tag?
The antibody is developed against a synthetic 14 aa peptide (GKPIPNPLLGLDST), the V5 tag, which presents in many vectors. A novel function of anaphase promoting complex subunit 10 in tumor progression in non-small cell lung cancer.
Are there any recombinant monoclonal antibodies for v5?
GKPIPNPLLGLDST (V5 epitope) conjugated with KLH. The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
Where does the V5 tag come from in simian virus?
The V5 epitope tag is derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus (SV5). The V5 tag is usually used with all 14 amino acids (GKPIPNPLLGLDST), although it has also been used with a shorter 9 amino acid sequence (IPNPLLGLD).
How many amino acids are in the V5 tag?
The V5 tag is usually used with all 14 amino acids (GKPIPNPLLGLDST), although it has also been used with a shorter 9 amino acid sequence (IPNPLLGLD). Lanes 1-3 : Cells transfected with v5-tagged protein. Lanes 4-5 : Untransfected cells.