Does Acinetobacter calcoaceticus ferment lactose?

Although officially classified as not lactose-fermenting, they are often partially lactose-fermenting when grown on MacConkey agar. They are oxidase-negative, catalase-positive, indole-negative, nonmotile, and usually nitrate-negative.

Does Acinetobacter baumannii grow on MacConkey Agar?

baumannii in infection control surveillance cultures of hospitalized patients. MacConkey agar may be reliable for isolating Acinetobacter species but it is non-selective for Acinetobacter and does not differentiate Acinetobacter from other non-lactose fermenting gram-negative bacteria.

Can Acinetobacter ferment glucose?

Acinetobacter spp. are nonmotile, oxidase-negative, catalase-positive, strictly aerobic, gram-negative coccobacilli. Their inability to ferment glucose or to reduce nitrate clearly separates these organisms from the family Enterobacteriaceae.

Does Acinetobacter grow on blood agar?

Acinetobacter isolates were tested for their abilities to oxidize glucose in Hugh and Leifson’s medium, cause browning of blood agar containing 0.22 M glucose, and grow on plain blood agar when they are incubated at 44°C.

How did Acinetobacter calcoaceticus get its name?

The first ‘ Acinetobacter ’ microorganisms were identified in 1911 by the Dutch microbiologist, Beijerinck, who assigned the bacteria the name Micrococcus calcoaceticus following their isolation from soil ( Beijerinck, 1911; Baumann et al., 1968 ). Subsequently, at least 15 other ‘generic’ names have been used to describe Acinetobacter.

How big are Acinetobacter colonies on nutrient agar?

On media such as nutrient agar and Trypticase Soy Agar, Acinetobacter form smooth, sometimes mucoid, pale yellow to greyish white colonies, about 1–2 mm in diameter. A selective medium for the growth of Acinetobacter is Herellea agar ( Mandel et al., 1964 ).

What kind of bacteria is pink on MacConkey?

Lactose fermenting bacteria or lactose + bacteria on MacConkey will appear as bright pink colonies. Non-lactose fermenting bacteria will be colorless (or, if they have any color, will be their natural color rather than pink). BAP or SBA: An abbreviation for blood agar plate or sheep bloog agar.

What is the colony morphology of Acinetobacter baumannii?

Colony morphology on nutrient agar, blood agar, and MacConkey agar plates has to be observed and documented. On nutrient agar, non-mucoid, opaque, and circular colonies will be observed. On blood agar, nonhemolytic, opaque, circular, and gray color colonies will be observed.