What is in buffered peptone water?
What is in buffered peptone water?
Buffered Peptone Water (BPW) is used for the non-selective pre-enrichment of Salmonella spp….Item No. NCM0003A.
| Formula | Liter |
|---|---|
| Enzymatic Digest of Casein | 10.0 g/L |
| Sodium Chloride | 5.0 g/L |
| Disodium Hydrogen Phosphate (anhydrous) | 3.5 g/L |
| Potassium Dihydrogen Phosphate | 1.5 g/L |
What is buffered peptone water and why is it used?
Oxoid Buffered Peptone Water may be used as a pre-enrichment medium, prior to selective enrichment in the isolation of salmonellae from foods. It also provides conditions for resuscitation of cells that have been injured by processes of food preservation.
Is peptone a buffer?
Buffered Peptone Water / 5kg Unlike other pre-enrichment formulations such as Lactose Broth, BPW contains phosphate salts that provide buffering capacity to maintain the pH of the enrichment. The peptone in BPW provides a carbon and nitrogen source, and sodium chloride maintains osmotic balance.
What is the use of peptone water?
Peptone Water is used as a growth medium and as a base for carbohydrate fermentation media. It is a broth medium used for the detection of indole. It is minimal growth medium which is used for determining carbohydrate fermentation patterns of non-fastidious organisms.
What is the purpose of buffered peptone water?
Buffered Peptone Water is a non-selective pre-enrichment medium. It also provides conditions for resuscitation of cells that have been injured by processes of food preservation. Low numbers and/or sub-lethally injured Salmonella spp. cells may be present in a test food sample.
What kind of pH does peptone water have?
Peptone water is a buffered solution generally containing peptone—a soluble protein—which is then buffered to a neutral pH (approximately 7.0) using a base disodium phosphate.
Is Thermo Scientific buffered peptone water safe to drink?
Pre-enrichment with Buffered Peptone Water maintained a high pH over a period of 24 hours of incubation. The vegetable tissue has a low buffering capacity and the medium overcame this problem. Not all products are available for sale in all territories. Please inquire. Remel™ and Oxoid™ products are now part of the Thermo Scientific brand.
Why are Salmonella cells present in buffered peptone water?
Low numbers and/or sub-lethally injured Salmonella spp. cells may be present in a test food sample. Pre-enrichment in non-selective Buffered Peptone Water allows the cells time to repair and multiply before being introduced into a selective culture, improving the chances of recovering Salmonella species from the sample.