What is the purpose of the 1Kb plus DNA ladder?

Invitrogen 1 Kb Plus DNA Ladder is designed for sizing and approximate quantification of double-stranded DNA in the range of 100 bp to 15,000 bp. 1 Kb Plus DNA Ladder consists of 18 individual chromatography-purified DNA fragments and has a reference band at 1,500 bp for easy orientation.

What does 1 kb ladder mean?

The 1 kb DNA ladder is a unique combination of a number of plasmids digested with restriction enzymes and PCR products to yield 13 DNA fragments that are suitable for use as a molecular weight standard for electrophoresis.

How do you dilute a 1Kb ladder?

The ladder is diluted to a 1:4 solution in water for use (3 parts water for 1 part ladder). To make 100 µl, 75 µl of water are combined with 25 µl of the DNA ladder. Then, 20 µl of 6X loading dye is added and the solution is split into 60 µl aliquots (0.5 ml microcentrifuge tubes) and stored at -20 C.

What fragments are present in a 1Kb ladder?

The ladder consists of 13 DNA fragments which are products of the restrictions of special plasmid by RsaI restrictase or products of PCR. All fragments are blunt-ended. The 1 kb and 3 kb fragments are present at increased intensity to allow easy identification.

How much is a DNA ladder?

For a standard electrophoresis system, we recommend loading 0.5 µg (20 µl) of the Fast DNA Ladder on the agarose gel. For a fast electrophoresis system (5 to 30 minutes separation), follow the system’s manufacturer recommendations: 5 to 20 µl load.

What does BP mean in DNA ladder?

DNA molecular weight (mw) standard controls of nucleic acids, also known as DNA ladders, are widely used in molecular biology studies to determine the mw or the base pair (bp) length of nucleic acids. The DNA ladder is also used to quantitatively analyze target DNA fragments.

How much DNA ladder should I load?

For a standard electrophoresis system, we recommend loading 0.5 µg (20 µl) of the Fast DNA Ladder on the agarose gel. For a fast electrophoresis system (5 to 30 minutes separation), follow the system’s manufacturer recommendations: 5 to 20 µl load. A dilution of the ladder may be required.

What is PCR and how is it used in DNA analysis?

The copying process, known as polymerase chain reaction (PCR), uses an enzyme (polymerase) to replicate DNA regions in a test tube. RFLP analysis requires a biological sample about the size of a quarter, but PCR can be used to reproduce millions of copies of the DNA contained in a few skin cells.

When to use generuler 1 kb DNA ladder?

In April 2018 this product was upgraded to include TriTrack DNA Loading Dye for better visualization. Thermo Scientific GeneRuler 1 kb DNA Ladder is recommended for sizing and approximate quantification of a double-stranded DNA in the range of 250 bp to 10,000 bp on agarose gels.

How big is a 100 bp DNA ladder?

Thermo Scientific GeneRuler 100 bp DNA Ladder is recommended for sizing and approximate quantification of a double-stranded DNA in the range of 100 bp to 1,000 bp on agarose or polyacrylamide gels. The DNA ladder consists of 10 DNA fragments and is provided with 6X TriTrack DNA Loading Dye.

Which is the best double stranded DNA ladder?

This double-stranded DNA ladder is compatible with 1% standard and precast agarose slab gels and can be visualized after ethidium bromide or SYBR Safe staining. A uniform intensity of bands and bright reference bands allow easy sizing and orientation. A defined amount of DNA in each band enables approximate quantification of sample DNA.

What kind of dye to use for DNA ladder?

The GelRed or GelGreen nucleic acid dyes can be used with our DNA Ladders. Nucleic acid binding dyes can affect DNA migration during electrophoresis, therefore we highly recommend post-staining of gels. Please follow the gel staining protocol provided by the manufacturer of nucleic acid gel dyes.