What is the principle of alkaline phosphatase?

This test is based on the principle that the alkaline phosphatase enzyme in raw milk liberates phenol from a disodium para-nitro phenyl phosphate and forms a yellow coloured complex at alkaline pH (Scharer, 1943). The intensity of yellow colour produced is proportional to the activity of the enzyme.

What is the specific activity of alkaline phosphatase?

ALP specific activity in the subpopulations ranged from 0.05 to 2.3 U/mg protein, and cytochemical analyses indicated multiple steady-state levels of ALP activity per cell.

What is alkaline phosphatase and its function?

Alkaline phosphatase is found in the periplasmic space of E. coli bacteria. This enzyme is heat stable and has its maximum activity at high pH. In humans, it is found in many forms depending on its origin within the body – it plays an integral role in metabolism within the liver and development within the skeleton.

What does alkaline phosphatase indicate?

ALP is an enzyme found throughout the body, but it is mostly found in the liver, bones, kidneys, and digestive system. When the liver is damaged, ALP may leak into the bloodstream. High levels of ALP can indicate liver disease or bone disorders.

What is the problem with alkaline phophatase?

High alkaline phosphatase levels may mean there is damage to your liver or that you have a type of bone disorder. Liver damage creates a different type of ALP than bone disorders do. If the test results show high alkaline phosphatase levels, your health care provider may order additional tests to find out where the extra ALP is coming from.

What does elevated alkaline phosphatase level mean?

IN THIS ARTICLE: Definition. The elevated alkaline phosphatase is a modification in the levels of alkaline phosphatase, which is a hydrolase enzyme responsible for the removal of phosphate from the different molecules of the body.

What is normal level of alkaline phosphatase?

Normal Values. The normal value for alkaline phosphatase is 53 to 128 U/L for a 20- to 50-year-old man and 42 to 98 U/L for a 20- to 50-year-old woman. Pregnant women typically have higher alkaline phosphatase values due to contributions from the placenta.

What causes high Alk Phos?

High alk. Phos can be caused by any/many liver disorders from any cause including hepatitis, obstruction of the bile due to stone or tumor.