What is pfu Turbo polymerase?

1 PfuTurbo DNA polymerase is a mixture of cloned Pfu DNA polymerase and the exclusive thermostable ArchaeMaxx polymerase-enhancing factor that enhances PCR product yields and increases target length capability without altering DNA replication fidelity. PfuTurbo DNA polymerase can be used to amplify complex genomic DNA.

What is Taq and pfu?

Unlike Taq DNA polymerase, Pfu DNA polymerase possesses 3′ to 5′ exonuclease proofreading activity, meaning that as the DNA is assembled from the 5′ end to 3′ end, the exonuclease activity immediately removes nucleotides misincorporated at the 3′ end of the growing DNA strand.

What is PFU buffer?

Description: Pfu DNA Polymerase is a thermostable enzyme that replicates DNA at 75°C. Enzyme Storage Buffer: Pfu DNA Polymerase is supplied in 50mM Tris-HCl (pH 8.2 at 25°C), 0.1mM EDTA, 1mM DTT, 0.05% CHAPS and 50% glycerol.

What makes Taq polymerase unique?

Taq DNA polymerase is the most common enzyme used for PCR amplification. This enzyme is extremely heat resistant with a half-life of 40 minutes at 95°C. This will result in the amplification of non-specific targets that can be overcome by the use of a ‘hot-start’ PCR technique (Mullis 1991).

What is the difference between Pfu polymerase and Taq polymerase?

The main difference between Pfu and alternative enzymes is that Pfu has superior thermostability and proof-reading properties. Unlike Taq DNA polymerase, Pfu DNA polymerase also possesses 3′->5′ exonuclease proofreading activity, resulting in PCR fragments with fewer errors than Taq-generated PCR inserts.

Is Pfu a high fidelity polymerase?

Pfu DNA Polymerase is a high-fidelity, thermostable enzyme of approximately 90kDa isolated from Pyrococcus furiosus. The enzyme replicates DNA at 75°C, catalyzing the polymerization of nucleotides in the 5´→3´ direction in the presence of magnesium.

What does PFU stand for?

plaque-forming unit
A plaque-forming unit (PFU) is a measure used in virology to describe the number of virus particles capable of forming plaques per unit volume.

What is the most important advantage of PFU polymerase over Taq polymerase?

What is the most important advantage of Pfu polymerase over Taq polymerase? Unlike Taq polymerase, Pfu polymerase has proofreading activity. Unlike Taq polymerase, Pfu polymerase functions well at relatively high temperatures. Pfu polymerase removes the need for primers during PCR.

What is PFU per ml?

The pfu/mL result represents the number of infective particles within the sample and is based on the assumption that each plaque formed is representative of one infective virus particle.

How is PFU different from Taq DNA polymerase?

Unlike Taq DNA polymerase, Pfu DNA polymerase possesses 3′ to 5′ exonuclease proofreading activity, meaning that as the DNA is assembled from the 5′ end to 3′ end, the exonuclease activity immediately removes nucleotides misincorporated at the 3′ end of the growing DNA strand.

How is PFU used in the PCR process?

In the laboratory setting, Pfu is used to amplify DNA in the polymerase chain reaction (PCR), where the enzyme serves the central function of copying a new strand of DNA during each extension step. Pfu DNA polymerase has superior thermostability and proofreading properties compared to Taq DNA polymerase.

Which is family of polymerases does Taq belong to?

Based on sequence homology to Escherichia coli DNA polymerase I, Taq DNA polymerase has been included in a family of DNA polymerases known as Family A ( Braithwaite and Ito, 1993 ).

What is the role of PfuTurbo DNA polymerase?

PfuTurbo DNA polymerase provides robust amplification of long, complex genomic targets. A key component of PfuTurbo DNA polymerase is the ArchaeMaxx polymerase-enhancing factor. The ArchaeMaxx factor eliminates a PCR inhibitor and promotes shorter extension times, higher yield, and greater target length capabilities.