What is Opti-MEM used for?

Opti-MEM® I Reduced-Serum Medium is an improved Minimal Essential Medium (MEM) that allows for a reduction of Fetal Bovine Serum supplementation by at least 50% with no change in growth rate or morphology.

Why Opti-MEM is used in transfection?

Opti-MEM is a serum-reduced media used during transfection of genetic molecules into mammalian cells. Similar amounts of knockdown of the target proteins were achieved in DMEM, and cell viability was higher compared with Opti-MEM after transfection.

Can cells grow in Opti-MEM?

Most cells grown in serum-supplemented media can be transferred to Opti-MEM media with a minimum of 50% reduction in serum. Opti-MEM I Reduced Serum Media is ideal for use during cationic lipid transfections—especially Invitrogen Lipofectamine transfection reagents.

Does optimem have biotin?

Since free biotin in the culture media might compete for streptavidin, consideration was given to culture media: Iscove’s modified Dulbecco’s medium (IMDM) contains 52.3 nM biotin, while DMEM contains none.

How is GIBCO Opti-Mem I reduced serum medium?

Gibco™ Opti-MEM™ I Reduced Serum Medium. An improved Minimal Essential Medium that allows for a reduction of Fetal Bovine Serum supplementation by at least 50% with no change in growth rate or morphology.

Where is the manufacturing facility for GIBCO Opti-Mem I?

Opti-MEM™ I is manufactured at a cGMP-compliant facility located in Paisley, Scotland, UK. The facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard.

What do you need to know about Opti Mem I medium?

These additional components allow for a reduction in serum supplementation by at least 50%. Opti-MEM® I medium uses a sodium bicarbonate buffer system (2.4 g/L), and therefore requires a 5–10% CO 2 environment to maintain physiological pH.

Is it OK to freeze Opti Mem I reduced serum medium?

Actually, we recommend storing this medium at 2-8 degrees C in the dark. We do not recommend freezing the medium because it may precipitate. Can the Gibco Media Configurator be used for my own proprietary formulation?