What is Oligofectamine?

Oligofectamine™ Transfection Reagent is a proprietary formulation for transfecting oligonucleotides and short interfering RNA (siRNA) into eukaryotic cells. Oligofectamine™ Reagent is suitable for nuclear and cytoplasmic targets and transfects a wide variety of cell lines including CHO, HEK-293, NIH 3T3, and HeLa.

What is Lipofectamine made of?

Lipofectamine consists of a 3:1 mixture of DOSPA (2,3‐dioleoyloxy‐N‐ [2(sperminecarboxamido)ethyl]‐N,N‐dimethyl‐1‐propaniminium trifluoroacetate) and DOPE, which complexes with negatively charged nucleic acid molecules to allow them to overcome the electrostatic repulsion of the cell membrane.

How long can you store Lipofectamine?

We guarantee the performance of the product, if stored and handled properly, for six months following purchase. We do not recommend freezing transfection reagents as this usually decreases transfection performance.

Is Lipofectamine 2000 toxic to cells?

I have found that in high concentrations, lipofectamine is toxic to HeLa cells, and as previously mentioned, the cell number seeded has an impact on this. I have good results from using DNA to Lipofectamine ratio of 1:1 and 1:2.

Does Lipofectamine expire?

This kit is shipped on wet ice. Lipofectamine™ 2000 Transfection Reagent should be stored at 40 C. the first week however following week it may not. Expired reagent is not necessarily unusable, but will likely give lowered transfection efficiency.

What is Lipofectamine reagent?

Lipofectamine® Transfection Reagent is one of our first-generation, cationic-lipid transfection reagents formulated for the transfection of DNA into eukaryotic cells. Lipofectamine® Transfection Reagent is a trusted, value reagent with many years of cited use in thousands of publications and with many cell lines.

What is the difference between lipofectamine 2000 and RNAiMAX?

Lipofectamine® rnaiMaX reagent is designed specifically for the delivery of sirna and mirna while Lipofectamine® 2000 reagent delivers Dna or sirna with excellent transfection performance for protein expression, gene silencing, and functional assays.

Is DNA toxic to cells?

An excess of plasmid DNA can also contribute to cellular toxicity, and through empirical testing we have determined that a good starting point is one microgram per well of a 12-well plate. This results in maximum protein expression as well as minimal toxicity.

What is the function of siRNA?

siRNAs. siRNAs are highly specific and usually synthesized to reduce the translation of specific messenger RNAs (mRNAs). This is done to reduce the synthesis of particular proteins. They form from double-stranded RNA transcribed and then cut to size in the nucleus before releasing into the cytoplasm.

How is Lipofectamine used in molecular biology?

Lipofectamine. Lipofectamine or Lipofectamine 2000 is a common transfection reagent, produced and sold by Invitrogen, used in molecular and cellular biology. It is used to increase the transfection efficiency of RNA (including mRNA and siRNA) or plasmid DNA into in vitro cell cultures by lipofection.

How is oligofectamine used in the transfection of cells?

Oligofectamine™ Reagent interacts spontaneously with oligonucleotides to form transfection complexes. Oligofectamine™ Reagent has been used successfully for the transfection of a wide variety of cultured eukaryotic cells including: NIH3T3, CHO, K562, HEK293, HeLaS3, and HeLa.

What is the transfection efficiencies of lipofectamine 3000?

Lipofectamine 3000, Lipofectamine 2000, and Lipofectamine LTX/PLUS provide excellent transfection efficiencies at confluencies between 70 and 90%. Some toxicity may be observed at lower confluencies but may be alleviated by decreasing quantity of complexes or removing the complexes after 4-6 hours incubation and refreshing the media.

How does Lipofectamine increase the transfection efficiency of RNA?

Lipofectamine. It is used to increase the transfection efficiency of RNA (including mRNA and siRNA) or plasmid DNA into in vitro cell cultures by lipofection. Lipofectamine contains lipid subunits that can form liposomes in an aqueous environment, which entrap the transfection payload, e.g. DNA plasmids.