How does DCFDA work?

DCFDA / H2DCFDA / DCFH-DA / DCFH is a fluorogenic dye that measures hydroxyl, peroxyl and other reactive oxygen species (ROS) activity within the cell. It is then deacetylated by cellular esterases to a non-fluorescent compound, which is later oxidized by ROS into 2′, 7′ –dichlorofluorescein (DCF).

What is DCFH-DA assay?

Dichloro-dihydro-fluorescein diacetate (DCFH-DA) assay: a quantitative method for oxidative stress assessment of nanoparticle-treated cells. Toxicol In Vitro.

How does DCFH-DA assay work?

DCFH-DA is the most widely used probe for detecting intracellular H2O2 and oxidative stress. This probe is cell-permeable and is hydrolyzed intracellularly to the DCFH carboxylate anion, which is retained in the cell.

How does cm-h2 DCFDA work in a cell?

CM-H 2 DCFDA passively diffuses into cells, where its acetate groups are cleaved by intracellular esterases and its thiol-reactive chloromethyl group reacts with intracellular glutathione and other thiols. Subsequent oxidation yields a fluorescent adduct that is trapped inside the cell, thus facilitating long-term studies.

Which is better for Ros cm or h2dcfda?

The chloromethyl derivative of H2DCFDA (CM-H2DCFDA) provides much better retention in live cells than H2DCFDA. Dead or dying cells produces ROS.

What does h2dcfda stand for in medical category?

H2DCFDA (Synonyms: DCFH-DA; 2′,7′-Dichlorodihydrofluorescein diacetate) H2DCFDA is a cell-permeable probe used to detect intracellular reactive oxygen species (ROS).

Which is better for oxidation h2dcfda or DCF?

Upon oxidation by ROS, th e nonfluorescent H2DCFDA is converted to the highly fluorescent 2′, 7′-dichlorofluorescein (DCF). The chloromethyl derivative of H2DCFDA (CM-H2DCFDA) provides much better retention in live cells than H2DCFDA. Dead or dying cells produces ROS.