Can proteins be detected by using oligonucleotide probes?

We developed a new nucleic acid-based fluorescence probe for protein detection. An oligonucleotide-based fluorescence probe was successfully applied to the detection of the ARM peptide in solution.

What is the purpose of probes?

A probe is a spacecraft that travels through space to collect science information. Probes do not have astronauts. Probes send data back to Earth for scientists to study.

How do DNA probes work?

Gene probes are small, single-stranded fragments of DNA that hybridize to target DNA sequences in a sample. Tagged with a label like color or fluorescence, they allow researchers to identify a specific sequence of DNA in a mixture. First, the DNA sample is heated to separate the DNA strands, then the probe is applied.

What is oligonucleotide primer?

The term oligonucleotide is derived from the Greek “oligo,” which means few or small. Oligonucleotides made up of 2′-deoxyribonucleotides are the molecules used in polymerase chain reaction (PCR). These are referred to as primers and are used to massively amplify a small amount of DNA.

How do oligonucleotide probes work?

Oligonucleotide probes are short stretches of single-stranded DNA or RNA used to detect the presence of complementary nucleic acid sequences (target sequences) by hybridization. Oligonucleotide probes are usually labelled, for example with radioisotopes, epitopes, biotin or fluorophores to enable their detection.

What is meant by oligonucleotide?

An oligonucleotide is a compound comprised of about three to twenty nucleotides. Each monomeric unit component is comprised of a nucleobase, a pentose moiety, and phosphate group. Oligonucleotides are short DNA or RNA molecules. They readily bind to complementary oligonucleotides or to nucleic acids, forming a duplex.

What is probe in PCR?

Probes are fluorescently labelled DNA oligonucleotides. They are designed to bind downstream of one of the primers during the PCR reaction and to give a fluorescent signal during the reaction. The 5′ end of the probe is labelled with a fluorescent reporter molecule.

How do radioactive probes work?

So radioactive DNA probes are basically single strands of DNA or RNA with a radioactive tag. Their sequence is usually the complementary of a single sequence of DNA that researchers want to find in an array of other DNA (such as a gene). They track it to see where it binds in the array of DNA.

What are called DNA probes?

A probe is a single-stranded sequence of DNA or RNA used to search for its complementary sequence in a sample genome. The probe is labeled with a radioactive or chemical tag that allows its binding to be visualized.

Are oligos primers?

Researchers use oligos that are anywhere between 20-35 bases long called primers to start copying or amplifying. This DNA primer is usually custom designed to match the target sequence of DNA for copying.

What are the types of probes?

There are three basic types of probe used in emergency and critical care point-of-care ultrasound: linear, curvilinear, and phased array. Linear (also sometimes called vascular) probes are generally high frequency, better for imaging superficial structures and vessels, and are also often called a vascular probe.

What kind of probes are used for oligonucleotides?

Improved portal to help customize your oligos with standard deoxynucleotides, modified bases, and 5′- and 3′-modified nucleotides, available in up to five quantities and four purity levels. Select dual-labeled probes and unlabeled sequence detection primers for real-time PCR applications using TaqMan probe-based chemistry.

What can oligonucleotides be used for in microarray?

In addition to their use as PCR primers, oligonucleotides are used as probes, in microarray, in situ hybridization, and antisense analyses, and even as drug carriers. DID YOU KNOW? Bio-Rad offers custom and pre-designed real-time PCR Primers.

Are there any restrictions on use of oligonucleotides?

Value oligos are excluded. Offer void where prohibited, licensed, or restricted by federal, state, provincial, or local laws or regulation or agency/institutional policy. Other restrictions may apply. For Research Use Only.

When do Life Technologies oligonucleotides go on sale?

* Open only to customers in the US (excluding Puerto Rico) and Canada. This offer will apply to qualifying orders received by Life Technologies no later than December 31, 2020, or until promotional supplies are depleted, whichever comes first. Value oligos are excluded.