Why is the FastDigest Green Buffer added to the DNA samples?

FastDigest enzymes in combination with FastDigest (Green) Buffer are designed to eliminate star activity: Short incubation time (5–15 min), no need for prolonged digestions. Universal buffer with optimal pH and salt concentration for all enzymes.

What is in FastDigest buffer?

The 10X FastDigest Green Buffer includes a density reagent and two tracking dyes for direct loading. The blue dye migrates with 3–5 kb DNA fragments in a 1% agarose gel and has an excitation peak of 424 nm. The yellow dye migrates faster than 10 bp DNA fragments in a 1% agarose gel and has an excitation peak of 615 nm.

What is FastDigest?

Description. The 10X FastDigest Buffer is a proprietary digestion buffer which supports 100% activity of all FastDigest restriction enzymes. DNA/RNA modifying enzymes, such as ligases, phosphatases, kinases and mesophilic DNA polymerases also have 100% activity in FastDigest Buffer.

What enzyme digests the fastest?

It’s that easy. Thermo Scientific FastDigest restriction enzymes are an advanced line of enzymes which offer: Complete digestion in 5-15 minutes. 100% buffer compatibility with downstream applications.

What kind of enzymes are used in FastDigest green buffer?

DNA/RNA modifying enzymes, such as ligases, phosphatases, kinases and mesophilic DNA polymerases also have 100% activity in FastDigest Green Buffer. Therefore, enzymes used in downstream applications can be directly added to the FastDigest reaction mix • Double and multiple digestion in a single buffer with any combination of enzymes.

How are FastDigest enzymes used in DNA digestion?

FastDigest enzymes are an advanced line of fast restriction enzymes that are all 100% active in the universal FastDigest and FastDigest Green reaction buffers. The universal buffer allows rapid single-, double- or multiple DNA digestion within 5-15 minutes eliminating any need for buffer change or subsequent DNA clean-up steps.

Are there two versions of the Universal FastDigest buffer?

Two versions of the universal FastDigest buffer are supplied with each enzyme: 10X FastDigest Buffer and 10X FastDigest Green Buffer. All FastDigest enzymes are 100% active in both buffers. Enzymes used in common downstream applications such as ligation, blunting and dephosphorylation also have 100% activity in both buffers.

How long to incubate Universal FastDigest green buffer?

Short incubation times and optimal composition of the universal FastDigest Buffer eliminate star activity effects. All FastDigest enzymes are 100% active in the universal FastDigest and FastDigest Green buffers and are able to digest DNA in 5-15 minutes.