What is the function of citrate synthase?

Citrate synthase catalyzes the Claisen condensation between acetyl CoA and oxaloacetate to yield, after hydrolysis of the thioester bond, citrate and CoA. This reaction probably occurs via the stabilized enolate anion of acetyl CoA.

What does citrate do in the mitochondria?

Citrate can activate the gluconeogenic enzyme fructose 1,6-bisphosphatase (FBPase1) and ACC, and so stimulate fatty acid synthesis. The mitochondrial citrate carrier (CIC), also known as solute carrier family 25 member 1 (Slc25a1), can export citrate from the mitochondria in exchange for malate (37).

How does citrate synthase work?

What is citrate synthase activated by?

Citrate synthase starts this process by taking the molecules of acetate and attaching them to oxaloacetate, which acts as a convenient handle as the carbon atoms are passed from enzyme to enzyme in the citric acid cycle.

Where is citrate synthase located in the mitochondria?

Citrate synthase locates in the matrix of the mitochondria. The integrity of the mitochondrial inner membrane is assessed by measuring citrate synthase activity in the presence and absence of the detergent, triton X-100.

What is the function of citrate synthase in skeletal muscle?

Citrate synthase is commonly used as a quantitative enzyme marker for the presence of intact mitochondria. Maximal activity of citrate synthase indicates the mitochondrial content of skeletal muscle.

How is citrate synthase used as a quantitative marker?

Citrate synthase. Citrate synthase is commonly used as a quantitative enzyme marker for the presence of intact mitochondria. Maximal activity of citrate synthase indicates the mitochondrial content of skeletal muscle. The maximal activity can be increased by endurance training or high-intensity interval training,…

Why is citrate synthase important to the TCA cycle?

The mutant enzymes were found to restore rates of mitochondrial respiration, citrate oxidation, and growth on acetate to near parental levels, suggesting that interactions among mitochondrial components, presumably in this case between the synthase and transporter, are critical for TCA cycle function. W.R. Driedzic,