What is flow cytometry PNH?

Flow cytometry is the gold standard in diagnosis of paroxysmal nocturnal hemoglobinuria (PNH) by detecting the absence of glycol-phosphatidyl inositol (GPI)-linked protein expression on granulocyte and monocyte surfaces.

What is the test for PNH?

Flow cytometry is the preferred technique for the diagnosis of PNH and is performed to evaluate for the presence of GPI-linked antigens on blood cells.

Is PNH a hemolytic anemia?

PNH is characterized by hemolytic anemia, bone marrow failure and a tendency toward the development of thrombosis.

Why does PNH cause venous thrombosis?

Platelet activation, complement-mediated hemolysis, impaired nitric oxide (NO) bioavailability, impairment of the fibrinolytic system, and inflammatory mediators are all proposed mechanisms and thought to be responsible for the increased thrombotic risk in patients with PNH.

When to use flow cytometric to diagnose PNH?

In the clinical setting of a patient with intravascular hemolysis, unexplained deep venous thrombosis, or pancytopenia,it is useful for the diagnosis of PNH. Because of its increased sensitivity and ability to analyze multiple cell lineages, it can detect the presence of PNH clones in AA patients in the absence of RBC involvement.

When to use flow cytometry for paroxysmal nocturnal hemoglobinuria?

Tabs Content. Once a patient has been assessed for hemolysis (see Initial Evaluation) and paroxysmal nocturnal hemoglobinuria (PNH) is suspected, the recommended test is flow cytometry to evaluate for the presence of glycosylphosphatidylinositol (GPI)-linked antigens on blood cells.

How is PNH used to diagnose hemolysis?

Laboratory testing for PNH is used to: Guidelines for the diagnosis and monitoring of paroxysmal nocturnal hemoglobinuria and related disorders by flow cytometry. Diagnosis and management of paroxysmal nocturnal hemoglobinuria. Unexplained hemolysis with iron deficiency, abdominal pain, thrombosis, dysphagia, or granulocytopenia/thrombocytopenia

How is flow cytometry used in the hematology lab?

The flow cytometric differential method we employed utilized strong expression of CD16 to highlight mature neutrophils. CD16 expression may be decreased or absent in neutrophils of patients with paroxysymal nocturnal hemoglobinuria (PNH) as CD16 is a GPI linked protein (see this months CSI case for more details about PNH).