What is an iBlot?

The iBlot 2 Gel Transfer Device is a dry transfer device that performs western blotting transfer efficiently and reliably, within seven minutes, and without the need for liquid buffers.

How do you transfer blot?

After electrophoresis is complete, proteins must be transferred from the gel onto a suitable membrane for antibody staining and detection. Transfer is performed by passing a current across the gel to the membrane. There are two common membrane types used for western blot analysis: PVDF and nitrocellulose.

Which gel is used in Western blotting?

Gel electrophoresis Western blot uses two different types of agarose gel: stacking and separating gel. The higher, stacking gel is slightly acidic (pH 6.8) and has a lower acrylamide concentration making a porous gel, which separates protein poorly but allows them to form thin, sharply defined bands.

How does the iblot 2 gel transfer device work?

The design of the iBlot 2 Gel Transfer Device reduces the distance between the electrodes and the integrated power supply. This unique design combined with the gel matrix technology of iBlot 2 Transfer Stacks allows the system to generate high field strength and increase the transfer speed.

How long does it take to blot with iblot 2?

Western blotting of proteins from midi – or mini-sized polyacrylamide gels onto nitrocellulose or PVDF membranes within 7 minutes can be performed with iBlot 2 Transfer Stacks . See the next page to understand how the iBlot

Is the iblot 2 system compatible with PVDF?

Enjoy fast western transfer without sacrificing efficiency and uniformity. The iBlot 2 system is compatible with both polyvinylidene difluoride (PVDF) and nitrocellulose membranes, and has comparable performance to traditional wet transfer methods in a fraction of the time.

How does the iblot dry blotting system work?

The iBlot®Gel Transfer Device is pre-programmed with 10 voltage programs listed below: 1. Open the lid of the device. 2. Unseal the Anode Stack, Bottom. Keep the stack in the plastic tray. 3. Place the Anode Stack (bot- tom; in the tray) on the blot- ting surface. Align it with the Gel Barriers on the right. 4.