What is 4/12 Bis-Tris gel?

Description. Invitrogen NuPAGE Bis-Tris protein gels are precast polyacrylamide gels designed to give optimal separation of a wide range of proteins under denaturing conditions. Unlike traditional Tris-glycine gels, NuPAGE Bis-Tris gels have a neutral pH environment that minimizes protein modifications.

How do you make BIS with Tris gel?

Mix: 1/3.5 vol. of 3.5X bis-Tris gel buffer (or 1/3 of 1 M), acrylamide to 8% (30:2.0) or 12-15% (27.5:1), and water to final volume. I make 3.75 mLs for each Bio-Rad Protean gel, and use 3.5 mLs per gel. (if you made a 3X stock, adjust the buffer component volume).

What is a 4/12 gel?

A variety of 4-12% gradient gels can be found from different suppliers for protein electrophoresis. Precast gradient gels are solid, polymerized acrylamide slabs with a gradually increasing concentration toward the bottom of the gel.

What is the purpose of SDS PAGE?

SDS-PAGE separates proteins primarily by mass because the ionic detergent SDS denatures and binds to proteins to make them uniformly negatively charged. Thus, when a current is applied, all SDS-bound proteins in a sample will migrate through the gel toward the positively charged electrode.

What is Tris-glycine used for?

Tris-Glycine gel chemistry The Tris-Glycine gel formulation for gel electrophoresis is the simplest and most widely used system for separating a broad range of proteins using SDS PAGE or native PAGE (i.e., without SDS or alternative denaturant).

What is a bis-Tris gel?

Invitrogen NuPAGE Bis-Tris protein gels are precast polyacrylamide gels designed to give optimal separation of a wide range of proteins under denaturing conditions. Unlike traditional Tris-glycine gels, NuPAGE Bis-Tris gels have a neutral pH environment that minimizes protein modifications.

What is the basic principle of SDS-PAGE?

The principle of SDS-PAGE states that a charged molecule migrates to the electrode with the opposite sign when placed in an electric field. The separation of the charged molecules depends upon the relative mobility of charged species. The smaller molecules migrate faster due to less resistance during electrophoresis.

What is a Tris-glycine gel?

Invitrogen Novex Tris-Glycine Gels are robust gels for a wide range of sample types and molecular weights. They offer sharp straight bands and easier-to-load, larger capacity wells, and can be used for denaturing or native PAGE applications.

What is tricine for?

Tricine is a commonly used electrophoresis buffer and is also used for the resuspension of cell pellets. It has a lower negative charge than glycine, allowing it to migrate faster. In addition, its high ionic strength causes more ion movement and less protein movement.

What are the advantages of SDS-PAGE?

SDS polyacrylamide gel electrophoresis (SDS-PAGE) has the advantages of simple operation and good reproducibility in the determination of protein molecular weight, detection of specific proteins, and identification of strain species.

Is the main function of SDS?

SDS (sodium dodecyl sulfate) is an anionic detergent that unfolds and denatures proteins, coating proteins in negative charge. It is added in excess to the proteins, so that the proteins’ intrinsic charge is covered, and a similar charge-to-mass ratio is obtained for all proteins.

How many Invitrogen gels are in a mini tank?

Separation Range: 3.5 to 260 kd; Separation Type: Denaturing; For Use With: Mini Gel Tank, XCell SureLock Mini For Research Use Only. All usage must comply with product instructions. Includes: Catalog numbers for gels refer to a single box. One box contains 10 gels.

How big is a nupage Bis tris gel?

NuPAGE Bis-Tris protein gels come in four polyacrylamide concentrations: 8%, 10%, 12%, and a 4–12% gradient. Gels come in two sizes: mini (8 cm x 8 cm) or midi (8.7 cm x 13.3 cm) and either 1.0 mm (mini and midi gels) or 1.5 mm (mini gel format only) in thickness. NuPAGE Bis-Tris gels also come in multiple well formats.

How long does 4 to 12% Bis tris last?

NuPAGE® Novex® 4-12% Bis-Tris Midi Gel w/MOPS. Bio-Rad Criterion™ XT 4-12% Bis-Tris Gel w/MOPS. One box contains 10 gels. Store at 4–25°C. Do not freeze. Shelf life is 12 months. Convenient, on-site access to the products you need. Learn more. No results found for your search criteria. No results found for your search criteria.

Can a 26 well gel work with a 12 well gel?

The 12-channel pipettes work with the 26-well gels. You will need to alternate lanes (e.g., aligns with every two lanes). There will be two extra lanes (for MW markers). The 12-channel pipettes work with the 12+2 well gels as well. Are your precast protein gels available in Mini and Midi formats?