How much chloroform do I add to TRIzol?

Add 0.2 ml of chloroform per 1 ml of TRIZOL Reagent.

Which step is the most critical in TRIzol RNA extraction?

homogenization
The most important step in extraction is homogenization and the ratio of Trizol and cells/tissue (see the document below). The concentration you obtain is fine, if you use little solvent. Check Your RNA 260/280 and 260/230 ratios, both should exceed 2.1 (ideally, >2.0 is more or less OK).

How much TRIzol do I add?

Add 0.75 mL of TRIzol™ Reagent per 0.25 mL of sample (5– 10 × 106 cells from animal, plant, or yeasty origin or 1 ×107 cells of bacterial origin) to the pellet. Note: Do not wash cells before addition of TRIzol™ Reagent to avoid mRNA degradation.

How do you use TRIzol for RNA extraction?

Precipitate the RNA from the aqueous phase by mixing with isopropyl alcohol. Use 0.5 ml of isopropyl alcohol per 1 ml of TRIZOL Reagent used for the initial homogenization. Incubate samples at 15 to 30°C for 10 minutes and centrifuge at no more than 12,000xg for 10 minutes at 2 to 8°C.

When to use Invitrogen TRIzol as a reagent?

Invitrogen™TRIzol Reagent is a ready-to-use reagent, designed to isolate high quality total RNA (as well as DNA and proteins) from cell and tissue samples of human, animal, plant, yeast, or bacterial origin, within one hour.

How is TRIzol used for RNA isolation in PCR?

The Invitrogen Life Technologies TRIzol Reagent (Total RNA Isolation Reagent) is a ready-to-use reagent for the isolation of total RNA from cells and tissues for use in PCR analysis. TRIzol reagent is a mono-phasic solution of phenol and guanidine isothiocyanate. During tissue homogenization or lysis, the TRIzol

What can TRIzol be used for in western blots?

• Isolated protein can be used for Western Blots, recovery of some enzymatic activity, and some immunoprecipitation. For RNA and protein isolation, refer to TRIzol™ Reagent User Guide (Pub. No. MAN0001271). TRIzol ™ Reagent can also be used with Phasemaker Tubes to isolate RNA.

Where can I get a safety data sheet for TRIzol?

Safety Data Sheets (SDSs) are available from thermofisher.com/support. Invitrogen ™ TRIzol Reagent is a ready-to-use reagent, designed to isolate high quality total RNA (as well as DNA and proteins) from cell and tissue samples of human, animal, plant, yeast, or bacterial origin, within one hour.