How is thymidine monophosphate formed?

In normal conditions, thymidylate synthetase converts dUMP into thymidine monophosphate using 5,10-methylenetetrahydrofolate (derived from folate) as a methyl group donor.

Which vitamin is necessary for the synthesis of thymidine monophosphate?

The conversion of deoxyuridine monophosphate (dUMP) to deoxythymidine monophosphate (dTMP) during DNA synthesis is by methyl group transfer, facilitated by both vitamin B12 and folate.

How is dTMP synthesized?

The de novo dTMP synthesis pathway catalyzes conversion of deoxyuridylate (dUMP) to dTMP, and involves four enzymes. Salvage synthesis occurs through the action of thymidine kinase (TK), which catalyzes the ATP-dependent phosphorylation of the nucleoside thymidine to the nucleotide dTMP.

How is deoxyuridine monophosphate converted to dTMP?

The conversion of deoxyuridine monophosphate (dUMP) to deoxythymidine monophosphate (dTMP) during DNA synthesis is by methyl group transfer, facilitated by both vitamin B 12 and folate. The other source of dTMP is via phosphorylation of deoxythymidine, catalysed by thymidine kinase, which is subject to feedback inhibition by its product, dTMP.

How is thymidine monophosphate used in DNA synthesis?

Thymidylate synthase (TS) catalyzes a crucial step in DNA synthesis by methylating deoxyuridine monophosphate (dUMP) to dTMP using 5,10-methylenetetrahydrofolate (5,10-CH2THF). dTMP is subsequently phosphorylated to produce dTTP, a vital precursor for DNA replication and repair (Wilson et al., 2014).

Which is a product of thymidylate synthase ( dTMP )?

Both cause DNA damage. The following reaction is catalyzed by thymidylate synthase: By means of reductive methylation, deoxyuridine monophosphate (dUMP) and N5,N10-methylene tetrahydrofolate are together used to form dTMP, yielding dihydrofolate as a secondary product.

Which is an enzyme that catalyzes the conversion of deoxyuridine monophosphate?

Thymidylate synthetase (EC 2.1.1.45) is an enzyme that catalyzes the conversion of deoxyuridine monophosphate (dUMP) to deoxythymidine monophosphate (dTMP). Thymidine is one of the nucleotides in DNA.