How does Janus green stain mitochondria?
How does Janus green stain mitochondria?
Janus green B (JG-B) dye is used for vital staining of mitochondria and its reduction and oxidation shows the electron transfer chain alteration. The defect in electron transfer chain of mitochondria by paraquat is linked to free radical formation.
What is role of Janus Green B in mounting of mitochondria?
Answer: Janus green is used to stain exclusively mitochondria. If this stain is not used, mitochondria can’t be distinguished from other cellular organelles. Janus Green B is a basic dye and vital stain used in histology.
What structures does Janus Green B stain in a cell?
Janus Green B should be oxidized to become colored. Thus, this dye can be used to specifically stain mitochondria in living cells. It has been used to stain biomolecules, nucleic acids and chromosomes.
How do you make Janus green B solution?
To prepare a Janus Green B solution, use a 1% aqueous solution. Add 1 g of Janus Green B to 75 ml of DI water. Dilute to a final volume of 100 ml with DI water.
What is the use of Janus green B solution?
Janus Green B is a basic dye and vital stain used in histology. It is also used to stain mitochondria supravitally, as was introduced by Leonor Michaelis in 1900. The indicator Janus Green B changes colour according to the amount of oxygen present. When oxygen is present, the indicator oxidizes to a blue colour.
What is the Colour of the Diazine Green B?
Answer: The colour of diazine green B is Blue.
Which stain is used for mitochondria?
Janus green stain
To observe mitochondria in the live cells, Janus green stain is used.
What is the use of Janus Green B?
What is the use of Diazine solution?
by Biology experts to help you in doubts & scoring excellent marks in Class 10 exams. Diazine green is added to glucose solution to know whether oxygen is present or not in glucose solution. When the availability of oxygen is less than the diazine green changes to pink colour.
What color do mitochondria stain?
Hematoxylin creates a violet color and primarily stains nucleic acid. Eosin is a pink-orange color and stains proteins.
What is the charge of Janus Green?
0
3.1Computed Properties
| Property Name | Property Value | Reference |
|---|---|---|
| Formal Charge | 0 | Computed by PubChem |
| Complexity | 694 | Computed by Cactvs 3.4.8.18 (PubChem release 2021.05.07) |
| Isotope Atom Count | 0 | Computed by PubChem |
| Defined Atom Stereocenter Count | 0 | Computed by PubChem |
What is the use of adding Diazine green to glucose solution?
What kind of dye is Janus Green B?
Janus Green B chloride is an organic chloride salt. It has a role as a dye. It contains a Janus Green B cation. 8- [ [4- (dimethylamino)phenyl]diazenyl]- N, N -diethyl-10-phenylphenazin-10-ium-2-amine;chloride
When to add ammonium sulfate to Janus Green B?
The desired quantity of dye having been added cubated in the presence of oxygen for 10 min at 38°C. The mix the unbound dye removed by centrifugation. All subsequent operations were performed at these temperatures. After adding line ammonium sulfate to 12 percent. After centrifuging the 1, 4, 10, 30, and 40 percent, respectively.
When did Janus Green B inoculate his mice?
The object of the present experiments atmosphere. white Swiss mice of about 25 gm body weight. The technic of inoculation has already been described (8). Tumor-dye mixture, Received October 28, 1965; accepted November 28, 1966. 0.15 ml, was inoculated in all animals.