How do you make a 4-OHT?

For injection of 4-OHT, dissolve 100 mg of 4-OHT powder in 1 mL of ethanol (final concentration of 100 mg/mL), and then dilute the solution 1/10 in peanut or corn oil.

How do you dissolve 4-OHT?

4-OHT Protocol 7-30-18 Before use, 4-OHT should be re-dissolved in ethanol by shaking at 37C for 15 min, a 1:4 mixture of castor oil:sunflower seed oil was added to give a final concentration of 10 mg/ml 4-OHT, and the ethanol should be evaporated by vacuum under centrifugation.

Is Tamoxifen soluble in blood?

Tamoxifen is soluble in organic solvents such as ethanol, DMSO, and dimethyl formamide (DMF), which should be purged with an inert gas. The solubility of tamoxifen in ethanol and DMF is approximately 20 mg/ml and approximately 2 mg/ml in DMSO. Tamoxifen is sparingly soluble in aqueous buffers.

How do you dissolve Hydroxytamoxifen?

Soluble in ethanol (20 mg/ml, with heating) and in methanol (10 mg/ml) producing clear faint yellow solutions. Solutions should be stored protected from light at –20 °C.

How is tamoxifen administered to mice?

The most used method is intraperitoneal injection, because the amount of administered compound can be better controlled, but delivery by oral gavage is also possible. For short-term and immediate-effect studies or when conversion of tamoxifen by the liver is to be avoided, 4-OHT can be used directly.

Is tamoxifen toxic to the liver?

Introduction. Tamoxifen is a nonsteroidal antiestrogen that is widely used in the treatment and prevention of breast cancer. Long term tamoxifen therapy has been associated with development of fatty liver, steatohepatitis, cirrhosis, and rare instances of clinically apparent acute liver injury.

What does tamoxifen do to the body?

Tamoxifen is a type of hormonal therapy known as a selective estrogen receptor modulator (SERM). The drug attaches to hormone receptors (specific proteins) in breast cancer cells. Once the medication is inside the cells, it stops the cancer from accessing the hormones they need to multiply and grow.

What is the half life of tamoxifen in mice?

Table 1

Is tamoxifen toxic to mice?

Tamoxifen treatments have been widely reported to produce toxicity or death in mice. This can include stomach, intestinal and liver cellular abnormalities.

Why is tamoxifen used in mice?

For adult mice, a standard dose of 100µl tamoxifen/corn oil solution (above) is effective for inducing recombination. Administer tamoxifen via intraperitoneal injection (using an ACUC approved injection procedure) once every 24 hours for a total of 5 consecutive days.

What are the long term side effects of tamoxifen?

Tamoxifen can have side effects, including hot flashes, fatigue, and an increased risk of blood clots and endometrial cancer. But there was no substantial increase in serious side effects, including endometrial cancer incidence or death, in women who took tamoxifen for the longer period, Gray reported.

Why is tamoxifen toxic?

Together, these data suggest that tamoxifen induces RPE cell death through multiple cell death pathways, as both necrosis and apoptosis appear to be activated following tamoxifen treatment. Necrotic and apoptotic cell death pathways are activated by tamoxifen toxicity.

Which is used in cell culture, tamoxifen or 4 OH?

4-OH tamoxifen is typically used in cell culture. 4-OH tamoxifen is the active form of the drug, tamoxifen gets converted to 4-OH tamoxifen in vivo. Okay. Thanks. Just one more query: In term of stability, I guess tamoxifen is more stable than 4-OH tamoxifen?

What is the metabolite of 4-hydroxytamoxifen?

Oncogene, 2016, 36 (16):2255-2264. 4-Hydroxytamoxifen (Afimoxifene) purchased from Selleck. 4-Hydroxytamoxifen (Afimoxifene) is the active metabolite of tamoxifen and a selective estrogen receptor (ER) modulator that is widely used in the therapeutic and chemopreventive treatment of breast cancer.

How is 4-hydroxytamoxifen used in breast cancer treatment?

For research use only. 4-Hydroxytamoxifen is the active metabolite of tamoxifen and a selective estrogen receptor (ER) modulator that is widely used in the therapeutic and chemopreventive treatment of breast cancer. It activates on-target modification frequency for intein-Cas9 variants. Next day delivery by 10:00 a.m. Order now.

Can you use tamoxifen free base in culture?

Popular Answers (1) For cells in culture I have used 4-hydroxy tamoxifen instead of tamoxifen free base. It’s more expensive than the free base, but worked very well in vitro. It is dissolved in methanol at 5mg/ml as a stock solution, which can be further diluted in PBS.