How do you isolate Streptomyces from soil?

In isolating actinomycetes, soil samples are collected from the study area [16-20]. To kill most of the Gram negative bacteria, the soil is dried on the bench inside the laboratory for a period of one week [17]. The soil is further held in a water bath at 50°C to further destroy other vegetative microorganisms [13].

How do you isolate antibiotics from the soil?

Because we want to isolate both bacterial and fungal antibiotic producers, two different kinds of media will be used. Glycerol yeast extract media is for isolation of Streptomyces, while Saboraud dextrose agar medium will be used for the isolation of the fungi.

Which method is used for detection of antibiotic producers from soil?

A modified agar overlay technique was developed for detecting antibiotic production by Streptomyces isolates and compared with two routinely used overlay procedures and the shake flask-disk assay method.

Which is the media used for the isolation of Streptomyces?

The choice of media for sporulation of Streptomyces, however, varies largely from species to species. Most Streptomyces species sporulate very well on Oatmeal-agar, MYM-agar and ISP4-agar media.

How is Streptomyces isolated from fresh water and soil?

This unit describes a general protocol for the isolation of Streptomyces species from soil and fresh water, using a procedure for the selective growth of Streptomyces species. Preparation of the necessary growth medium, recognition of the morphology of the bacteria, and safety considerations are also covered. Content may be subject to copyright.

How many antibiotics are produced by Streptomyces species?

Streptomyces species are Gram-positive, aerobic microorganisms with high DNA G + C contents and produce about half of all known antibiotics from microorganisms [15]. The species belonging to the genus Streptomyces constitutes 50% of the total population of soil actinomycetes and 75–80% of the commercially and medicinally useful antibiotics [16].

How many Streptomyces isolates are there in the world?

A comparative analysis using the statistical software Minitab shows that 67 of these isolates are presumably new strains, since they possess a very low percentage of similarity with other reported species. Only four isolates shared 100% identity with one, two or three reported Streptomyces spp.

How many Streptomyces spp.are there in Venezuela?

They differ greatly in their morphology, physiology, and biochemical activities, producing the majority of known antibiotics. The morphological and biochemical characteristics of 71 Streptomyces spp. isolated from soil samples collected at different places of Venezuela, are presented.