What is the purpose of RNA-binding proteins?

RNA-binding proteins (RBPs) have important functions in the regulation of gene expression. RBPs play key roles in post-transcriptional processes in all eukaryotes, such as splicing regulation, mRNA transport and modulation of mRNA translation and decay.

What are RNA-binding motifs?

RNA-binding proteins (often abbreviated as RBPs) are proteins that bind to the double or single stranded RNA in cells and participate in forming ribonucleoprotein complexes. RBPs contain various structural motifs, such as RNA recognition motif (RRM), dsRNA binding domain, zinc finger and others.

How many proteins are in RNA-binding?

This approach identified 88 mostly highly abundant proteins, of which 22 were known RBPs. Identification of RNA-binding protein repertoires in vivo by RNA interactome capture.

How do you identify RNA-binding proteins?

Alternatively, the protein may be labeled, or the RNA–protein complex may be isolated using an antibody against the protein of interest. The RNA is then detected by northern blot or through RT-PCR analysis and the proteins detected by western blotting or mass spectrometry.

Are RBP binding sites required for splicing?

In situations of such restricted competition, RBP binding would be necessary to promote exon inclusion by enhancing the downstream intron splicing pathway (Figure 4b).

What is RNA pulldown?

RNA pull down is a popular RNA-centric approach to study RNA-protein interaction. RNA pull down assay depends on the principle that streptavidin agarose bead has a strong affinity to biotin-labeled RNA, and can effectively and specifically pull down biotinylated RNA–protein complexes.

How do you study RNA-protein interaction?

Researchers can use protein–RNA cross-linking to identify in vivo interactions by purifying the RNA under denaturing conditions that remove noncovalent interactions, and subsequently extracting only the cross-linked proteins for identification.

Is RNA made from DNA?

RNA is synthesized from DNA by an enzyme known as RNA polymerase during a process called transcription. The new RNA sequences are complementary to their DNA template, rather than being identical copies of the template. RNA is then translated into proteins by structures called ribosomes.

Why does gene splicing work?

Gene splicing is an important source of protein diversity. During a typical gene splicing event, the pre-mRNA transcribed from one gene can lead to different mature mRNA molecules that generate multiple functional proteins. In human cells, about 40-60% of the genes are known to exhibit alternative splicing.

What is the process of RNA splicing?

RNA splicing is a process that removes the intervening, non-coding sequences of genes (introns) from pre-mRNA and joins the protein-coding sequences (exons) together in order to enable translation of mRNA into a protein. From: Handbook of Clinical Neurology, 2014.

What does RBM24 RNA binding motif protein 24 do?

(RBM24 = RNA binding motif protein-24; CHRM2 = cholinergic receptor muscarinic 2) RBM24 interacted with the 5′ TR of HBV pregenomic RNA to block 80S ribosome assembly on HBV pgRNA and thus inhibited core protein translation, whereas the interaction between RBM24 and the 3′ TR enhanced the stability of HBV RNA.

How to identify target RNA motifs for RNA-binding proteins?

This study describes an HuR target RNA motif and presents a general strategy for identifying target motifs for RNA-binding proteins.

How is the Hur motif used in RNA prediction?

Computational analysis of the strongest HuR targets on the array allowed us to delineate the requirements of primary sequence and secondary structure in the HuR target motif. The HuR motif described here was successfully used in the prediction of novel HuR target mRNAs. Cell Culture and Preparation of Lysates.

How does RBM24 bind to CHRM2 coding region?

Data suggests that RBM24 binds to coding region of CHRM2 to regulate mRNA stability in cardiomyocytes; RBM24appears to drive changes in alternative splicing and in production of alternative transcript isoforms. (RBM24 = RNA binding motif protein-24; CHRM2 = cholinergic receptor muscarinic 2)