What is the principle of estimation of aspirin?

Principle and Procedure: The amount of aspirin can be determined by brominating using KBrO3-KBr mixture. A definite amount of aspirin is refluxed with NaOH. Then salicylic acid is formed. The excess brominating mixture formed is titrated with standard thio.

Which indicator is used in assay of aspirin?

Phenolphthalein
Phenolphthalein is an indicator because it changes color when all the aspirin has been reacted. This is called the “end point” of the reaction. If one continues adding NaOH after the end point, the solution will turn dark red.

How does aspirin mechanism of action?

The acetyl group of acetylsalicylic acid binds with a serine residue of the cyclooxygenase-1 (COX-1) enzyme, leading to irreversible inhibition. This prevents the production of pain-causing prostaglandins.

What is the purpose of synthesis of aspirin?

The purpose of this lab was to synthesize Aspirin and measure the synthesized Aspirin’s purity. By calculating the theoretical yield based on the original amount of Salicylic acid, one could determine the actual yield percentage of the reaction.

What is the formula of aspirin?

C₉H₈O₄
Aspirin/Formula

What is the role of ethanol in assay of aspirin?

water — the ethanol helps the aspirin dissolve. Note that an aspirin tablet contains other compounds in addition to aspirin. The aspirin/NaOH acid-base reaction consumes one mole of hydroxide per mole of aspirin.

What are the pharmacological action of aspirin?

Aspirin causes several different effects in the body, mainly the reduction of inflammation, analgesia (relief of pain), the prevention of clotting, and the reduction of fever. Much of this is believed to be due to decreased production of prostaglandins and TXA2.

What are the mechanism of action and adverse effect of aspirin?

He proved that aspirin and other non-steroid anti-inflammatory drugs (NSAIDs) inhibit the activity of the enzyme now called cyclooxygenase (COX) which leads to the formation of prostaglandins (PGs) that cause inflammation, swelling, pain and fever.

What type of reaction is synthesis of aspirin?

The synthesis of aspirin is known in organic chemistry as an esterification reaction. This is a substitution reaction in which an alcohol (the –OH group in salicylic acid) reacts with acetic anhydride to form an ester, aspirin.

What is aspirin and its structure?

Acetylsalicylic acid is prototypical analgesic with a chemical formula C9H8O4. It is also known as aspirin or 2-Acetoxybenzoic acid. It appears as a crystalline powder which is colourless to white. Generally, it has no smell but when in moist air it acquires a smell of acetic acid. It has a flashpoint of 482° F.

Is aspirin acidic or basic?

Aspirin itself is an acidic medicine and causes gastric irritation and regurgitation which can lead to low oral pH levels [7].

Is aspirin an acid or base?

How to determine the concentration of aspirin using back titration?

Determination of Aspirin using Back Titration This experiment is designed to illustrate techniques used in a typical indirector backtitration. You will use the NaOH you standardized last week to back titrate an aspirin solution and determine the concentration of aspirin in a typical analgesic tablet. You will be graded on your accuracy.

How is the aspirin and P2Y12 response assay used?

The assays enable platelet function testing for patients taking aspirin, P2Y12 inhibitors (clopidogrel, ticlopidine, or prasugrel), and IIb/IIIa inhibitors (abciximab or eptifibatide) and has demonstrated nearly 100% sensitivity and 96% specificity for the detection of antiplatelet function.

How to calculate the theoretical yield of aspirin?

Weigh 4.0 g (0.030 mol) of salicylic acid in a 125 mL Erlenmeyer flask. Using this quantity of salicylic acid to calculate the theoretical yield of aspirin. Record the weigh on the report sheet. 2. Carefully add 6 mL (0.051 mol) of acetic anhydride to the flask. (CAREFUL! Acetic anhydride is irritating to the skin and eyes.) 3.

How to do colorimetric analysis of aspirin content?

Colorimetric analysis of aspirin content in a commercial tablet v010214 Objective In this lab, you will prepare standard solutions, and use Beer’s Law to construct a calibration curve. You will determine molar absorptivity, and use your calibration curve to determine the aspirin content in a commercial preparation. Background