What is Lowenstein-Jensen media used for?

Löwenstein-Jensen medium, better known as LJ medium, is a selective egg-based medium specifically used for the culture and isolation of Mycobacterium species, including Mycobacterium tuberculosis, from clinical specimens.

How long does Mycobacterium tuberculosis grow on Lowenstein-Jensen medium?

Samples are inoculated on to Lowenstein-Jensen medium and incubated at 37 °C for about two to three months. Human and bovine strains of M. tuberculosis take about six to eight weeks to produce recognizable growth, while colonies of atypical mycobacteria can be recognized after two to seven days.

What disease is caused by Mycobacterium avium?

MAC lung disease is an infection caused a group of bacteria called Mycobacterium avium complex (MAC). MAC includes two closely related species, Mycobacterium avium and Mycobacterium intracellulare, and may also be referred to as MAI.

Is LJ medium complex or defined?

Lowenstein-Jensen (LJ) is the selective medium which is used for the cultivation and isolation of Mycobacterium species.

What kind of medium is Lowenstein-Jensen used for?

Lowenstein-Jensen (LJ) is the selective medium which is used for the cultivation and isolation of Mycobacterium species. It was developed by Lowenstein who incorporated congo red and malachite green to inhibit unwanted bacteria.

Which is the best selective medium for Mycobacterium?

Lowenstein-Jensen (LJ) is the selective medium which is used for the cultivation and isolation of Mycobacterium species. It was developed by Lowenstein who incorporated congo red and malachite green to inhibit unwanted bacteria. The present formulation, a glycerated egg-based medium, is based upon Jensen’s modification.

Why is malachite green added to Lowenstein-Jensen?

Jensen’s version eliminates congo red and uses a moderate concentration of malachite green to prevent growth of the majority of contaminants surviving decontamination of the specimen. This formulation also encourages the earliest possible growth of mycobacteria. For cultivation of M. bovis, glycerol is omitted and sodium pyruvate is added.

What are the ingredients of Lowenstein-Jensen LJ?

Composition of LJ Medium Ingredients Amount Magnesium Citrate 0.6 gm Malachite Green 0.4 gm Magnesium Sulfate 0.24 gm Glycerol 12 ml