What is isolectin?

Isolectin IB4 is a 114 kDa glycoprotein and part of a family of five tetrameric type I isolectins (IA4, IA3B, IA2B2, IAB3, and IB4) isolated from the seeds of the tropical African legume Griffonia simplicifolia (formerly Bandeiraea simplicifolia).

What is Isolectin B4 used for?

The α-d-galactosyl-specific isolectin B4 (IB4) of Griffonia simplicifolia, an African medicinal plant, is a lectin widely used to label microglial cells in the rat, guinea pig, sheep, salamander, and even leech.

What does isolectin B4 stain?

Griffonia simplicifolia Isolectin B4 Identifies a Specific Subpopulation of Angiogenic Blood Vessels Following Contusive Spinal Cord Injury in the Adult Mouse.

What does IB4 stain for?

What is the binding target? Isolectin IB4 is used to for endothelial staining.

How does Isolectin work in the endothelial cells?

I am attaching one pdf file with the list of useful markers for endothelial cells. Isolectin binds to the sugar residues of the glycocalyx in the blood vessels. We used it to visualise the blood vessels and it works very well when injected into the circulation (iv).

What can GSL i-b4 Isolectin be used for?

Click “Submit”. GSL I-B4 isolectin contains only the B subunits. It is a useful marker for endothelial cells from nonprimates such as mouse, rat, rabbit, and goat as well as a marker for non-peptidergic unmyelinated primary afferent neurons.

What kind of endothelial cells does GSL I-B 4 contain?

GSL I-B 4 isolectin contains only the B subunits. It is a useful marker for endothelial cells from nonprimates such as mouse, rat, rabbit, and goat as well as a marker for non-peptidergic unmyelinated primary afferent neurons.

What kind of dye is used for Isolectin IB4?

Isolectin-IB4 was purchased with an Alexa 488 dye conjugate and did not require secondary antibody treatment (Molecular Probes, Eugene, OR). RECA-1, a ubiquitous marker of endothelial cells (1:25, mouse anti-RECA-1; Serotec, Raleigh, NC), was also used in this step in a separate experiment.