What is a recombinant plasmid?

A plasmid is a small, circular, double-stranded DNA molecule that is distinct from a cell’s chromosomal DNA. Researchers can insert DNA fragments or genes into a plasmid vector, creating a so-called recombinant plasmid. This plasmid can be introduced into a bacterium by way of the process called transformation.

What does para R plasmid do?

The plasmid pKAN-R carries the kanamycin resistant gene, kanr, which encodes a phosphotransferase, an en- zyme that transfers a phosphate group to the kanamycin molecule destroying its antibiotic effects. Kanamycin is an antibiotic that kills bacteria by preventing them from making proteins.

How is recombinant plasmid obtained?

Recombinant plasmid formation involves construction of rDNA, in which a foreign DNA fragment is inserted into a plasmid vector. 14.22 is inactivated upon insertion of the foreign DNA fragment illustrated by jigsaw pieces (Fig. 14.22).

What happens when the recombinant plasmid is digested with EcoRI?

A plasmid vector is digested with EcoRI at a single site to produce two sticky ends. The two samples are mixed and allowed to hybridize, some molecules will form with pieces of human DNA inserted into the plasmid vector at the EcoRI site. DNA ligase is used to covalently link the fragments.

What are the your factors in a plasmid?

R plasmid or R factors R plasmid is another group of important plasmids, also known as resistance factors , carries genes for antibiotics resistance. R factors contains genes that code for enzymes which have the capability to destroy or modify antibiotics.

What kind of plasmid is used in recombinant DNA?

A plasmid that was widely used in many recombinant DNA projects is pBR322 (Figure 3.3. 2). It replicates from an origin derived from a colicin-resistance plasmid (ColE1). This origin allows a fairly high copy number, about 100 copies of the plasmid per cell.

How many copies of plasmid pBR322 are there?

This origin allows a fairly high copy number, about 100 copies of the plasmid per cell. Plasmid pBR322 carries two antibiotic resistance genes, each derived from different transposons. These transposons were initially found in R-factors, which are larger plasmids that confer antibiotic resistance. Figure 3.3. 2: Features of plasmid pBR322.

What are the genes in a col plasmid?

Col plasmid contains genes for the synthesis of bacteriocins known as colicins. This plasmids codes for colicins, the proteins that kill sensitive coli cells, it also carry gene that provide immunity to the particular colicins. They are also known as bacteriocinogenic plasmid as they produces bacteriocins.