What are NHBE cells?

MatTek’s Normal Human Bronchial Epithelial Cells (NHBE) provide an ideal serum-free culture system to study cell-cell and cell-matrix interactions, drug effects, gene regulation, cell differentiation, tissue development, wound healing, inflammation and toxic effects to the bronchial epithelium.

What are BEAS 2B cells?

The BEAS-2B cell line was originally established as a human non-tumorigenic lung epithelial cell line derived from a human lung tissue and has been extensively used as an in vitro non-tumorigenic lung epithelial model in a large variety of studies in association with lung carcinogenesis for over 30 years.

Where is the epithelium?

Epithelial tissues are widespread throughout the body. They form the covering of all body surfaces, line body cavities and hollow organs, and are the major tissue in glands.

How are nhbe cells seeded in Ali culture?

NHBE cells were seeded on polyester Transwell inserts, followed by an ALI culture from day 3, which resulted in peak TEER value of 766+/- … Serially passaged normal human bronchial epithelial (NHBE) cell monolayers were established on Transwell inserts via an air-liquid interface (ALI) culture method.

How are nhbe cells cultured at passage two?

NHBE cells at passage two were cultured at ALI for 4 weeks in different growth media. The resulting 3D cultures were stained with Hematoxylin and Eosin (H&E). ( A) H&E staining of NHBE layers grown from cells cultured in M1 + ROCK (BEGM + ROCK), M2 (PnC Ex) or M3 (PnC-Ex-PLUS).

How are nhbe cells expanded in growth media?

Primary NHBE cells were originally expanded in three different growth media at passage one – BEGM (M1), PneumaCult-Ex (M2) or PneumaCult-Ex Plus (M3) (Fig. 1 and Fig. S1 ).

Is the ciliary beat frequency maintained in nhbe cells?

No change in ciliary beat frequency (CBF) or airway surface liquid (ASL) meniscus length was observed up to passage six. For the first time, this study demonstrates that CFTR ion channel function and normal epithelial phenotypic characteristics are maintained in passaged primary NHBE cells.