How do you do a 100 fold dilution?

For a 1:100 dilution, one part of the solution is mixed with 99 parts new solvent. Mixing 100 µL of a stock solution with 900 µL of water makes a 1:10 dilution. The final volume of the diluted sample is 1000 µL (1 mL), and the concentration is 1/10 that of the original solution.

What does a dilution factor of 100 mean?

DF=VfVi=10.0mL 0.1mL =100. You have diluted the sample by a factor of 100. The dilution factor is often used as the denominator of a fraction. For example, a DF of 100 means a 1:100 dilution.

How do you do a 200 fold dilution?

200 (1/200 dilution) = the first step in the dilution factor; the second is 1/50, obtained as follows: 1 ml of serum + 199 ml of diluent = 1/200 dilution. 1 ml of 1/200 dilution + 49 ml of diluent = 1/50.

How do you calculate 1 100 dilution factor?

In a serial dilution the total dilution factor at any point is the product of the individual dilution factors in each step up to it. Final dilution factor (DF) = DF1 * DF2 * DF3, etc. 1:100 * 100 = 1:10,000. Repeated again (the third step) the total dilution would be 1:100 * 10,000 = 1:1,000,000 total dilution.

How do you calculate dilution in chemistry?

The total dilution ratio can be determined by multiplying the dilution factor of each step leading up to the final step. This can be mathematically illustrated with the equation D t = D 1 x D 2 x D 3 x … x D n where D t is the total dilution factor and D n is the dilution ratio.

How do you calculate dilation factor?

To calculate the dilution factor, you need two things: the original volume of the solution you dilute and the final volume after diluting (or the volume you have added to dilute, in which case the final volume will be the original volume plus the volume you have added).

How do you calculate serial dilutions?

A serial dilution is any dilution in which the concentration decreases by the same factor in each successive step. In serial dilutions, you multiply the dilution factors for each step. The dilution factor or the dilution is the initial volume divided by the final volume. #DF = V_i/V_f# = #(1″mL”)/(10″mL”) = 1/10#. This is a 1:10 dilution.