What is the role of APS and TEMED?

Description. Thermo Scientific Pierce Ammonium Persulfate (APS) is an oxidizing agent that is used with TEMED to catalyze the polymerization of acrylamide and bisacrylamide to prepare polyacrylamide gels for electrophoresis.

What is TEMED biology?

Thermo Scientific Pierce Tetramethylethylenediamine (TEMED) is an essential catalyst for polyacrylamide gel polymerization. TEMED is used with ammonium persulfate (APS) to catalyze acrylamide polymerization when preparing gels for electrophoresis.

Why is APS and TEMED added last?

While making gel solution, TEMED has to be added last since it will immediately start to react with APS, and catalyze the polymerization of acrylamide and bisacrylamide. As a consequence, the following mixing and casting steps have to be completed as quickly as possible.

What is TEMED in SDS PAGE?

TEMED is an essential catalyst for polyacrylamide gel polymerization. It is used with ammonium persulfate (APS) to catalyze acrylamide polymerization when preparing gels for electrophoresis.

How is TEMED used in life science research?

TEMED is frequently used with another catalyst, APS, for the preparation of polyacrylamide gels for the protein and nucleic acid analysis. An extensive range of Acrylamide Solutions and Powders, in addition to Crosslinking Reagents and Catalysts are available. This product contains electrical or electronic material.

What does Bio-Rad do for Science Education?

For more than 20 years, Bio-Rad has made science education a major priority. To support this effort, the company has implemented a discount policy that allows K–12 and college teaching laboratories to purchase kits, instruments, reagents, and other equipment at preferred prices.

What kind of catalyst is TEMED used for?

Use TEMED (tetramethylethylenediamine) as a catalyst for the polymerization of acrylamide and bis-acrylamide. TEMED is frequently used with another catalyst, APS, for the preparation of polyacrylamide gels for the protein and nucleic acid analysis.

Why is TEMED used as a free radical stabilizer?

TEMED, is a free radical stabilizer. Free radicals promote acrylamide polimerization, and APS (ammonimum persulfate) which is other component of SDS gels, is a source of them. So the role of TEMED is stabilize these free radicals in order to improve the acrylamide polimerization. I hope this information is helpful for you.