What is the purpose of peptide mapping?

Peptide mapping is an identity test for proteins, especially those obtained by rDNA technology. It involves the chemical or enzymatic treatment of a protein, resulting in the formation of peptide fragments, followed by separation and identification of the resultant fragments in a reproducible manner.

What is the difference between G-CSF and Gmcsf?

Glycoproteins with a molecular weight of ~ 23 kDa, G-CSF and GM-CSF are now produced through recombinant technology in either E. coli or yeast. G-CSF induces the appearance of colonies containing only granulocytes, while GM-CSF gave colonies containing both granulocytes and macrophages.

What is the function of G-CSF?

G-CSF (granulocyte-colony stimulating factor) is a type of drug called a growth factor. It increases the number of some types of blood cells in the blood. It can be used with chemotherapy. It can also be used before and after a stem cell transplant.

Why is peptide mapping important in protein characterization?

Biopharmaceutical peptide mapping. Peptide mapping is a critical workflow in biotherapeutic protein characterization and is essential for elucidating the primary amino acid structure of proteins. For recombinant protein pharmaceuticals, such as monoclonal antibodies (mAbs) and antibody-drug conjugates…

How is peptide mapping done in mass spectrometry?

FIGURE 1. Peptide mapping for protein identification using mass spectrometry (MS). In the first step, the protein is proteolytically digested (usually with trypsin) and the experimental masses of the peptides are measured using MS.

How are cysteine residues used in peptide mapping?

Peptide map analysis of a protein containing two or more cysteine residues typically employs reduction and alkylation chemistry for efficient, reliable proteolysis, reproducible chromatographic profiles, and straightforward characterization by MS.

How long does it take for peptide mapping?

Some technologies currently employed for biopharmaceutical peptide mapping are subject to high levels of irreproducibility, poor sensitivity, and high levels of time-consuming manual work—with protracted methodologies that are not amenable to automation and often require 24 hours to achieve full protein digestion.