What is QF-PCR test?

QF-PCR analysis of microsatellite markers is widely used for rapid diagnosis of common aneuploidies and triploidy in prenatal, fetal tissue and newborn blood samples; aneuploidy mosaicism, maternal cell contamination and zygosity are also identified.

Can PCR detect aneuploidy?

QF-PCR is a molecular-based technique useful for rapid aneuploidy detection (RAD), and is increasingly being used in PND due to its ability to provide information in a cost-effective and timely manner [Badenas et al., 2010].

How is QF-PCR done?

QF-PCR is based on the amplification of chromosome-specific DNA sequences (STR, short tandem repeats) polymorphic in length between subjects. By means of fluorescent primers, the amplified segments can be visualized and quantified as peak areas on automated DNA scanners.

How is PCR used in prenatal testing to help diagnose fetal abnormalities?

Conventional PCR amplifies any DNA present in a sample and produces many copies of any DNA present. Therefore, if researchers used conventional PCR on maternal plasma samples, the resulting DNA may be maternal or fetal DNA. That makes the detection of rare genetic abnormalities harder.

Can a QF-PCR be used for sex chromosome testing?

These aspects of prenatal diagnosis are taken into account in these guidelines. implementation of a rapid aneuploidy diagnostic service by QF-PCR. Testing for trisomies 13, 18 and 21 shoul d be routinely carried out. It is acceptable to test for sex chromosome consultation with cl inicians and other relevant groups.

How are molecular methods used to diagnose aneuploidy?

Rapid molecular biological methods for prenatal diagnosis of the most common aneuploidies, collec tively known as rapid aneuploidy testing, are compared in this review. We discuss methodological problems and limitations of these various methods. All these techniques are believed to be accurate and carry a low risk of ing.

How big should an aliquot be for QF-PCR?

Generally between 0.5 and 4 ml (or 1/10 of the sample) is used for QF- PCR analysis, though it is noted that the larger aliquot sizes suggested may compromise subsequent karyotype analysis, particularly for early gestation/small samples. The size of the aliquots taken routinely from samples is a local decision.

When does QF-PCR fail the Resul T?

They arise when a mutation occurs wi thin the mi crosatellite usually involving an increase or decrease of 1-2 repeats. compared with the unaffected allel e (C). When this (A+B)/C pattern gives a ratio in the normal range, the marker result may be failed and not contribute to the resul t. with CPA standards for accredited l aboratories.