What is ODS HPLC column?

An ODS column is filled with a packing of octadecylsilyl groups (ODS groups or C18 groups) chemically bonded to a silica gel carrier. ODS columns are used for reverse-phase chromatography. ODS columns find a wide range of applications due to their high theoretical plate number and rapid equilibration.

Which column is best for HPLC?

A Quick Look at Reversed Phase HPLC

• C18 HPLC columns are the most preferred as they offer an excellent range of hydrophobic separation power along with high surface area coverage.
• C8 HPLC columns are used when less retention compared to a C18 is needed.

What is the difference between C8 and C18 HPLC columns?

C18 has 18 carbon atoms while C8 has only 8 carbon atoms. C18 has a longer carbon chain, but C8 has a shorter one. C18 has higher retention while C8 has shorter retention. C18 has higher hydrophobicity, but C8 has a lower hydrophobicity.

How many types of columns are there in HPLC?

High performance liquid chromatography (HPLC) is a type of liquid chromatography that uses a liquid moblie phase. The same basic principals from gas chromatography are applied to liquid chromatography. There are three basic types of liquid chromatographic columns: liquid-liquid, liquid-solid, and ion-exchange.

What is normal phase HPLC used for?

Normal phase chromatography, an adsorptive mechanism, is used for the analysis of solutes readily soluble in organic solvents, based on their polar differences such as amines, acids, metal complexes, etc.. Reversed-phase chromatography, a partition mechanism, is typically used for separations by non-polar differences.

Is C18 column polar or nonpolar?

A C18 column is an example of a “reverse phase” column. Reverse phase columns are often used with more polar solvents such as water, methanol or acetonitrile. The stationary phase is a nonpolar hydrocarbon, whereas the mobile phase is a polar liquid.

What is polar and nonpolar in HPLC?

Principle. In normal-phase chromatography, the stationary phase is polar and the mobile phase is nonpolar. In reversed phase we have just the opposite; the stationary phase is nonpolar and the mobile phase is polar. Retention increases as the amount of the polar solvent (water) in the mobile phase increases.

Which column is used in HPLC?

Different Types of HPLC Columns Used in Analysis

• Normal Phase Columns.
• Reverse Phase Columns.
• Ion Exchange Columns.
• Size Exclusion Columns.

Which is the ODS column for inertsil C18?

Inertsil ODS columns are general purpose, reversed phase C18 columns available in 5 μm and 10 μm particle sizes. It was the first ODS bonded phase introduced from GL Sciences back in 1986. We recommend InertSustain C18 or Inertsil ODS-4 columns for all new method development.

Which is the best column for inertsil HPLC?

Inertsil ODS-P Inertsil WP300 C18 Bonding density of ODS group is very high. As its steric selectivity is quite high, this column is useful for separation of similar compounds. It has larger pore on the surface of silica than ordinary columns. High- molecular-weight compounds can be eluted as sharp peaks.

What is the polar group of inertsil ODS-EP?

Inertsil ODS-EP contains a polar functional group embedded between the silica surface and the C18 group. The embedded polar group makes the C18 phase stable in 100 % aqueous eluents without “phase collapse.”

How is the carbon load controlled in inertsil?

The carbon load is controlled to elute highly hydrophobic analytes in shorter time. Contains a polar group embedded between an ODS group and silica. Compared with ordinary ODS columns, this column often delivers unique selectivity. Inertsil ODS-P Inertsil WP300 C18 Bonding density of ODS group is very high.