What is MST protein?

MicroScale Thermophoresis (MST) is a powerful technique to quantify biomolecular interactions. It is based on thermophoresis, the directed movement of molecules in a temperature gradient, which strongly depends on a variety of molecular properties such as size, charge, hydration shell or conformation.

What is KD confidence?

Kd is the dissociation constant or binding affinity, and c (target) is the final concentration of the target in the assay.

How do you Analyse MST data?

To analyze binding by MST, click on the MST button in the Choose Analysis Type panel. In cases of ligand-induced fluorescence changes where the fluorescence values of each capillary are used to determine binding constants, click the Initial Fluorescence button.

What affects protein binding affinity?

Binding affinity is influenced by non-covalent intermolecular interactions such as hydrogen bonding, electrostatic interactions, hydrophobic and Van der Waals forces between the two molecules. In addition, binding affinity between a ligand and its target molecule may be affected by the presence of other molecules.

How is MST used in biomolecular interaction studies?

By combining the precision of fluorescence detection with the variability and sensitivity of thermophoresis, MST provides a flexible, robust and fast way to dissect molecular interactions. In this review, we present recent progress and developments in MST technology and focus on MST applications beyond standard biomolecular interaction studies.

Are there any MST experts at 2bind?

The MST experts at 2bind were successfull in setting up various binding assays, stoichiometry assays, and competition assays and helped us to drive our science. We are very satisfied with the low sample consumption and the fast turnaround time. During several years we have had excellent experience working with 2bind.

How are protein binding assays used in drug development?

Specific interactions between proteins have a fundamental role in cellular functions and are important for biotechnological applications and antibody-based assays. In the following, we apply MST in several typical experiments to show its feasibility for use in interaction studies in the course of drug development or pharmaceutical research.

What kind of proteins do drugs bind to?

Drugs may bind to a wide variety of plasma proteins, including albumin. If the percentage of protein-bound drug is greater when measured in human blood than in a simple albumin solution, the clinician should suspect that the agent may be bound in vivo to one of these “minority” plasma proteins.