What is in Accutase?

A: Accutase contains no mammalian or bacterial components. It is a natural enzyme mixture with proteolytic and collagenolytic enzyme activity. This means it mimics the action of trypsin and collagenase at the same time.

What does Accutase cleave?

Accutase has been developed to meet the most demanding needs for gentle and effective detachment of adherent cells, ad fibroblasts, endothelial cells, neurons, tumor cell lines, and insect cells. Accutase gently and rapidly dissociates tissues for cell isolation and propagation.

Does Accutase contain trypsin?

Accutase replaces Trypsin/EDTA for the detachment and dissociation of anchorage-dependent cells from surfaces and can also be used on suspension cells to reduce clumping in preparation for counting.

How do you detach cells with Accutase?

Add an adequate volume of Accutase® Cell Detachment Solution to cover cells. 4. Allow cells to detach at room temperature or 37°C for 5 to 10 minutes, or until the cells are detached. 5.

How do you inactivate Accutase?

Leaving a bottle in a 37°C water bath until it is visibly defrosted could degrade the performance of Accutase® as it will be inactivated after 1 hour at 37°C. It is therefore recommend that Accutase® be defrosted overnight in the refrigerator or in a bath of cold water.

What is Matrigel used for?

A gelatinous protein mixture derived from mouse tumor cells and commercialized as Matrigel is commonly used as a basement membrane matrix for stem cells because it retains the stem cells in an undifferentiated state.

How is trypsin deactivated?

Trypsinization is the process of cell dissociation using trypsin, a proteolytic enzyme which breaks down proteins, to dissociate adherent cells from the vessel in which they are being cultured. When added to a cell culture, trypsin breaks down the proteins which enable the cells to adhere to the vessel.

Are detached cells dead?

These cell colonies are usually a bunch of cells. When they are dispersed/single, it means they are either dead or on the verge of death.

What does trypsin bind to?

Trypsin is a medium size globular protein that functions as a pancreatic serine protease. This enzyme hydrolyzes bonds by cleaving peptides on the C-terminal side of the amino acid residues lysine and arginine.

How many cells does a 6 well plate have?

Useful information for various sizes of cell culture dishes and flasks

How do you lift macrophages?

Using the teflon coated plates and placing on ice for 20-30 minutes is usually a successful manner in which to harvest adherant macrophages. Tapping the plate several times and pipetting is generally all you need to do to remove them.

How do you use Matrigel?

Add 200 μL of Matrigel matrix (8 to 11 mg/mL) into each well of a pre-chilled 24-well plate, spread evenly with a pipet tip, and then incubate at 37°C for 30 min. to allow the Matrigel matrix to gel. Note: All cultureware or media coming in contact with Matrigel matrix should be pre-chilled/ice-cold.

What kind of solution is accutase for cell detachment?

Accutase™ is a cell detachment solution comprised of collagenolytic and proteolytic enzymes and does not contain mammalian or bacterial derived products. Accutase™ is a replacement for trypsin solution and can be used for the passaging of cell lines.

When do you use accutase in suspension cells?

Accutase replaces Trypsin/EDTA for the detachment and dissociation of anchorage-dependent cells from surfaces and can also be used on suspension cells to reduce clumping in preparation for counting.

How is accutase used in cell culture media?

Accutase is particularly useful for detaching cells grown in cell culture media that does not contain serum, including Serum Free Media and Protein Free Media.

What does accutase stand for in medical category?

ACCUTASE is a trademark of Innovative Cell Technologies, Inc. STEMCELL TECHNOLOGIES INC.’S QUALITY MANAGEMENT SYSTEM IS CERTIFIED TO ISO 13485. PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED.