What is HLB sorbent?

Chromatography Columns. Oasis® HLB cartridges contain the Oasis® HLB sorbent, which is a universal polymeric reversed phase sorbent that was developed for the extraction of a wide range of acidic, basic, and neutral compounds from various matrices using a simple, generic protocol.

What is SPE column?

Pre-packed solid phase extraction columns, with a variety of sorbents or resins, for vacuum, positive pressure, or centrifugation processing methods; in a variety of particle sizes and bed weights.

What is C18 cartridge?

The C18 solid phase extraction (SPE) cartridge uses octadecyl silica as a filler to retain non-polar compounds by strong hydrophobic interaction. The filler retains most of the organic matter and is widely used in the fields of environment and food safety.

What is the main objective of solid phase extraction?

Analytical laboratories use solid phase extraction to concentrate and purify samples for analysis. Solid phase extraction can be used to isolate analytes of interest from a wide variety of matrices, including urine, blood, water, beverages, soil, and animal tissue.

What is an HLB column?

Universal Sorbent for Acidic, Neutral, and Basic Compounds Oasis® HLB is a Hydrophilic-Lipophilic-Balanced, water-wettable, reversed-phase sorbent for all your SPE needs. It is made from a specific ratio of two monomers, the hydrophilic N-vinylpyrrolidone and the lipophilic divinylbenzene.

What is Oasis wax?

Oasis WAX is designed to provide superior sample preparation for strong acids. It is a weak-anion-exchange, mixed-mode, water- wettable, polymeric sorbent, stable from pH 0 to 14. Rugged and highly selective SPE methods using Oasis WAX detect, confirm, and quantify strongly acidic compounds in biological fluids.

What are types of extraction?

Types of extraction

• Liquid–liquid extraction.
• Solid-phase extraction.
• Acid-base extraction.
• Supercritical fluid extraction.
• Ultrasound-assisted extraction.
• Heat reflux extraction.
• Mechanochemical-assisted extraction.
• Maceration.

What is SPE used for?

Solid-phase extraction (SPE) is a sample preparation technique routinely used in analytical laboratories for the extraction of analytes from a complex matrix. This sample preparation technique enables the extraction, cleanup and concentration of analytes prior to their quantification.

What are c4 C8 and C18 columns?

C8 and C18 both refer to the alkyl chain of a bonded face of a column. They are both used in the high performance liquid chromatography system. On the other hand, Octylsilane(C8) has only 8 carbon atoms on the column parking bonded to silica (Si). C18 will tend to retain more than C8.

What is C18 Sep Pak?

Sep-Pak C18 Vac cartridges contain a hydrophobic, reverse-phase, silica-based bonded phase that is used to adsorb analytes of even weak hydrophobicity from aqueous solutions. The Sep-Pak C18 sorbent is made using the most reproducible bonding technology available, utilizing monofunctional silane chemistry.

What is SPE method?

Solid phase extraction (SPE) is a technique designed for rapid, selective sample preparation and purification prior to the chromatographic analysis (e.g. HPLC, GC, TLC). In SPE, one or more analytes from a liquid sample are isolated by extracting, partitioning, and/or adsorbing onto a solid stationary phase.

What are the advantages of solid phase extraction?

What are advantages of doing Solid Phase Extraction?

• Cleaner Samples: In contrast with a liquid-liquid extraction where it only allows you to target classes of compounds, SPE enables you to target just your analyte of interest.
• Batch Processing: The ability to batch process multiple samples is another great advantage.

What is the purpose of the oasis HLB method?

The Oasis HLB method is a generic protocol used to isolate a broad range of acidic, basic, and neutral compounds from a wide spectrum of matrices. The Oasis 2×4 method for selection of SPE sorbent and protocol for acids, bases, and neutrals produces the cleanest extracts by eliminating matrix interferences and sample variability.

How does the oasis HLB 96-well µelution plate work?

Combining the Waters patented plate design, proven chemistries of the Oasis line, and straightforward protocols, the Oasis HLB 96-well µElution plate will help your lab to deliver high analyte recovery and clean extracts, even in volumes as low as 25 µL.

What kind of sorbent is in Oasis HLB?

Oasis HLB Cartridges and 96-Well Plates I. OASIS HLB Universal Sorbent for Acidic, Neutral, and Basic Compounds Oasis®HLB is a Hydrophilic-Lipophilic-Balanced, water-wettable, reversed-phase sorbent for all your SPE needs. It is made from a specific ratio of two monomers, the hydrophilic N-vinylpyrrolidone and the lipophilic divinylbenzene.

What do you need to know about Oasis HLB cartridges?

Adjust the initial extract to optimize analyte retention onto the SPE sorbent. This can include pH adjustment, solvent adjustment, or solvent exchange through evaporation and reconstitution (refer to Sections IV and V). It may be necessary to centrifuge or filter the sample prior to loading. c. Aqueous Samples: Water, Beverages